Clock gene expression and locomotor activity predict death in the last days of life in Drosophila melanogaster.

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Show simple item record Zhao, Jia en Warman, Guy en Cheeseman, James en 2019-09-23T23:03:52Z en 2018-08-09 en
dc.identifier.citation Scientific reports 8(1):11923 09 Aug 2018 en
dc.identifier.issn 2045-2322 en
dc.identifier.uri en
dc.description.abstract The importance of the circadian clock for the regulation of behaviour and physiology, and the molecular control of these rhythms by a set of clock genes are well defined. The circadian clock deteriorates with advancing age but the mechanism underlying is unclear. Here we recorded the expression of two key clock genes in young, middle-aged and old Drosophila using transgenic luciferase lines reporting period and timeless in vivo. We report a novel marker of imminent death in the expression of TIMELESS. In the days immediately preceding death TIMELESS expression increased to at least 150% of previous acrophase values (88.0% of n = 217) and lost circadian rhythmicity, which predicted death equally well in flies of different ages and under light and temperature cycles. We suggest this transient aberrant clock-gene expression is central to the mechanism of the disturbance in circadian behaviour before death (82.7% of n = 342). We also find that PERIOD expression in central-clock neurons remained robust with age, however PERIOD and TIMELESS in peripheral clocks showed a reduction in both expression level and rhythmicity. In conclusion, as flies age the molecular clock gradually declines at the peripheral level but continues to function at the central until days before death. en
dc.format.medium Electronic en
dc.language eng en
dc.relation.ispartofseries Scientific reports en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.rights.uri en
dc.subject Animals en
dc.subject Animals, Genetically Modified en
dc.subject Drosophila melanogaster en
dc.subject Drosophila Proteins en
dc.subject Survival Analysis en
dc.subject Gene Expression Regulation, Developmental en
dc.subject Circadian Rhythm en
dc.subject Aging en
dc.subject Longevity en
dc.subject Locomotion en
dc.subject Photoperiod en
dc.subject CLOCK Proteins en
dc.subject Period Circadian Proteins en
dc.subject Circadian Clocks en
dc.title Clock gene expression and locomotor activity predict death in the last days of life in Drosophila melanogaster. en
dc.type Journal Article en
dc.identifier.doi 10.1038/s41598-018-30323-x en
pubs.issue 1 en
pubs.begin-page 11923 en
pubs.volume 8 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.elements-id 751940 en Medical and Health Sciences en School of Medicine en Anaesthesiology en
dc.identifier.eissn 2045-2322 en
pubs.record-created-at-source-date 2018-08-11 en
pubs.dimensions-id 30093652 en

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