Protection of axonal integrity with 48 or 72 h of cerebral hypothermia in near-term fetal sheep

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dc.contributor.author Zhou, Kelly en
dc.contributor.author Draghi, V en
dc.contributor.author Lear, Christopher en
dc.contributor.author Dean, Justin en
dc.contributor.author Ashton, Jesse en
dc.contributor.author Hou, Y en
dc.contributor.author Bennet, Laura en
dc.contributor.author Gunn, Alistair en
dc.contributor.author Davidson, Joanne en
dc.date.accessioned 2019-09-26T21:35:33Z en
dc.date.issued 2019-06-24 en
dc.identifier.citation Pediatric research 9 pages 24 Jun 2019 en
dc.identifier.issn 0031-3998 en
dc.identifier.uri http://hdl.handle.net/2292/47947 en
dc.description.abstract Therapeutic hypothermia is partially protective for neonatal hypoxic-ischemic encephalopathy (HIE). Damage to the white matter tracts is highly associated with adverse outcomes after HIE, but the effectiveness and optimal duration of hypothermia to attenuate axonal injury are unclear. Near-term fetal sheep were randomized to sham control or cerebral ischemia for 30 min with normothermia or cerebral hypothermia from 3 to either 48 or 72 h. Sheep were killed after 7 days. SMI-312-labeled axons and myelin basic protein were quantified in the intragyral white matter of the first and second parasagittal gyri. Ischemia was associated with reduced axonal and myelin area fraction (p < 0.05); loss of axonal and myelin linearity (p < 0.05); and thin, sparse axons, with spheroids, compared to dense, linear morphology in sham controls and associated with induction of microglia in an amoeboid morphology. Both ischemia-48 h hypothermia and ischemia-72 h hypothermia improved axonal area fraction and linearity (p < 0.05), although abnormal morphological features were seen in a subset. Microglial induction was partially suppressed by ischemia-48 h hypothermia, but with a ramified morphology. These data suggest that therapeutic hypothermia can alleviate post-ischemic axonopathy, in part by suppressing secondary inflammation. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Pediatric research en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri https://www.nature.com/nature-research/editorial-policies/self-archiving-and-license-to-publish en
dc.title Protection of axonal integrity with 48 or 72 h of cerebral hypothermia in near-term fetal sheep en
dc.type Journal Article en
dc.identifier.doi 10.1038/s41390-019-0475-8 en
pubs.volume online first en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Article en
pubs.elements-id 775438 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Physiology Division en
dc.identifier.eissn 1530-0447 en
pubs.record-created-at-source-date 2019-07-01 en
pubs.dimensions-id 31234193 en


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