Evaluation of 2 ex vivo Bovine Cornea Storage Protocols for Drug Delivery Applications.

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dc.contributor.author Thakur, Sachin en
dc.contributor.author Shrestha, Darshan en
dc.contributor.author Rupenthal, Ilva en
dc.date.accessioned 2019-09-29T21:34:42Z en
dc.date.issued 2019-01 en
dc.identifier.issn 0030-3747 en
dc.identifier.uri http://hdl.handle.net/2292/47963 en
dc.description.abstract The use of corneal tissue for ex vivo therapeutic evaluations is limited due to its rapid loss of viability after excision. Optimization of storage conditions may allow prolonged retention of physical tissue properties. In this study, we evaluated how storage in optimized organ culture (OC) medium at 37°C or phosphate-buffered saline (PBS) at 2-8°C impacted physical properties of bovine corneas. Tissue hydration, permeability and histology were monitored at baseline and following 1, 4 and 7 days of storage. Corneas stored in OC demonstrated significantly higher hydration and permeability when compared to those stored in PBS. Histology revealed that storage in OC consistently caused detachment of the epithelial layer by day 4 of storage, whereas both storage conditions caused a significant increase in stromal thickness and tissue vacuolation. This study highlights the limitations of currently available corneal tissue storage approaches for ex vivo drug permeation studies. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Ophthalmic research en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Cornea en
dc.subject Animals en
dc.subject Cattle en
dc.subject Fluorescein en
dc.subject Culture Media en
dc.subject Tissue Preservation en
dc.subject Drug Delivery Systems en
dc.title Evaluation of 2 ex vivo Bovine Cornea Storage Protocols for Drug Delivery Applications. en
dc.type Journal Article en
dc.identifier.doi 10.1159/000493488 en
pubs.issue 4 en
pubs.begin-page 204 en
pubs.volume 61 en
dc.rights.holder Copyright: The author en
pubs.end-page 209 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Journal Article en
pubs.elements-id 757436 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Pharmacy en
pubs.org-id School of Medicine en
pubs.org-id Ophthalmology Department en
dc.identifier.eissn 1423-0259 en
pubs.record-created-at-source-date 2018-11-23 en
pubs.dimensions-id 30466090 en

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