Efficient synthesis and characterisation of the amyloid beta peptide, Aβ1-42, using a double linker system.

Kasim, Johanes; Kavianinia, Iman; Ng, Jin; Harris, Paul WR; Harris, Paul; Brimble, Margaret

dc.contributor.author	Kasim, Johanes	en
dc.contributor.author	Kavianinia, Iman	en
dc.contributor.author	Ng, Jin	en
dc.contributor.author	Harris, Paul WR	en
dc.contributor.author	Harris, Paul	en
dc.contributor.author	Brimble, Margaret	en
dc.date.accessioned	2019-09-29T21:34:45Z	en
dc.date.issued	2018-12	en
dc.identifier.citation	Organic & biomolecular chemistry 17(1):30-34 Dec 2018	en
dc.identifier.issn	1477-0520	en
dc.identifier.uri	http://hdl.handle.net/2292/47964	en
dc.description.abstract	The amyloidogenic Aβ42 peptide was efficiently prepared using a double linker system, markedly improving solubility and chromatographic peak resolution, thus enabling full characterisation using standard techniques. The tag was readily cleaved with sodium hydroxide and removed by aqueous extraction, affording Aβ42 in high purity and yield for biophysical characterisation studies.	en
dc.format.medium	Print	en
dc.language	eng	en
dc.relation.ispartofseries	Organic & biomolecular chemistry	en
dc.rights	Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.	en
dc.rights.uri	https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm	en
dc.rights.uri	https://creativecommons.org/licenses/by/3.0/	en
dc.subject	Humans	en
dc.subject	Sodium Hydroxide	en
dc.subject	Peptide Fragments	en
dc.subject	Chromatography, High Pressure Liquid	en
dc.subject	Staining and Labeling	en
dc.subject	Solubility	en
dc.subject	Amyloid beta-Peptides	en
dc.subject	Liquid-Liquid Extraction	en
dc.title	Efficient synthesis and characterisation of the amyloid beta peptide, Aβ1-42, using a double linker system.	en
dc.type	Journal Article	en
dc.identifier.doi	10.1039/c8ob02929f	en
pubs.issue	1	en
pubs.begin-page	30	en
pubs.volume	17	en
dc.rights.holder	Copyright: The Royal Society of Chemistry	en
pubs.end-page	34	en
pubs.publication-status	Published	en
dc.rights.accessrights	http://purl.org/eprint/accessRights/OpenAccess	en
pubs.subtype	Journal Article	en
pubs.elements-id	757439	en
pubs.org-id	Science	en
pubs.org-id	Biological Sciences	en
pubs.org-id	Chemistry	en
pubs.org-id	Science Research	en
pubs.org-id	Maurice Wilkins Centre (2010-2014)	en
dc.identifier.eissn	1477-0539	en
pubs.record-created-at-source-date	2018-12-01	en
pubs.dimensions-id	30500032	en