dc.contributor.author |
Radcliff, Fiona |
en |
dc.contributor.author |
Waldvogel-Thurlow, Sharon |
en |
dc.contributor.author |
Clow, Fiona |
en |
dc.contributor.author |
Mahadevan, Murali |
en |
dc.contributor.author |
Johnston, James |
en |
dc.contributor.author |
Li, Gen |
en |
dc.contributor.author |
Proft, Thomas |
en |
dc.contributor.author |
Douglas, Richard |
en |
dc.contributor.author |
Fraser, John |
en |
dc.date.accessioned |
2019-09-29T21:35:05Z |
en |
dc.date.issued |
2019-06-27 |
en |
dc.identifier.citation |
Pathogens 8(3) 27 Jun 2019 |
en |
dc.identifier.issn |
2076-0817 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/47969 |
en |
dc.description.abstract |
Staphylococcus aureus and Group A Streptococcus (GAS) are common occupants of the tonsils and many strains produce potent exotoxins (mitogens) that directly target T cells, which could be a driver for tonsillar hyperplasia. Tonsil tissues from 41 patients were tested for these bacteria in conjunction with profiling of B and T cells by flow cytometry. S. aureus and GAS were detected in tonsil tissue from 44% and 7%, respectively, of patients by bacteriological culture; immuno-histology showed bacteria in close proximity to both B and T lymphocytes. The presence of tonsillar S. aureus did not alter B or T cell populations, whereas peripheral blood mucosal-associated invariant T (MAIT) cells were significantly increased in S. aureus culture positive individuals (p < 0.006). Alterations of tonsil CD4+ TCR Vβ family members relative to peripheral blood were evident in 29 patients. Three patients had strong TCR Vβ skewing indicative of recent exposure to superantigens, their tonsils contained mitogenic bacteria, and supernatants from these bacteria were used to partially recapitulate the skewing profile in vitro, supporting the notion that superantigens can target tonsillar T cells in situ. Tonsils are a reservoir for superantigen-producing bacteria with the capacity to alter the composition and function of key immune cells. |
en |
dc.format.medium |
Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Pathogens (Basel, Switzerland) |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
en |
dc.title |
Impact of Superantigen-Producing Bacteria on T Cells from Tonsillar Hyperplasia. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.3390/pathogens8030090 |
en |
pubs.issue |
3 |
en |
pubs.volume |
8 |
en |
dc.rights.holder |
Copyright: The authors |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
research-article |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
775984 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Surgery Department |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
2076-0817 |
en |
pubs.record-created-at-source-date |
2019-06-30 |
en |
pubs.dimensions-id |
31252586 |
en |