Impact of Superantigen-Producing Bacteria on T Cells from Tonsillar Hyperplasia.

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Show simple item record Radcliff, Fiona en Waldvogel-Thurlow, Sharon en Clow, Fiona en Mahadevan, Murali en Johnston, James en Li, Gen en Proft, Thomas en Douglas, Richard en Fraser, John en 2019-09-29T21:35:05Z en 2019-06-27 en
dc.identifier.citation Pathogens 8(3) 27 Jun 2019 en
dc.identifier.issn 2076-0817 en
dc.identifier.uri en
dc.description.abstract Staphylococcus aureus and Group A Streptococcus (GAS) are common occupants of the tonsils and many strains produce potent exotoxins (mitogens) that directly target T cells, which could be a driver for tonsillar hyperplasia. Tonsil tissues from 41 patients were tested for these bacteria in conjunction with profiling of B and T cells by flow cytometry. S. aureus and GAS were detected in tonsil tissue from 44% and 7%, respectively, of patients by bacteriological culture; immuno-histology showed bacteria in close proximity to both B and T lymphocytes. The presence of tonsillar S. aureus did not alter B or T cell populations, whereas peripheral blood mucosal-associated invariant T (MAIT) cells were significantly increased in S. aureus culture positive individuals (p < 0.006). Alterations of tonsil CD4+ TCR Vβ family members relative to peripheral blood were evident in 29 patients. Three patients had strong TCR Vβ skewing indicative of recent exposure to superantigens, their tonsils contained mitogenic bacteria, and supernatants from these bacteria were used to partially recapitulate the skewing profile in vitro, supporting the notion that superantigens can target tonsillar T cells in situ. Tonsils are a reservoir for superantigen-producing bacteria with the capacity to alter the composition and function of key immune cells. en
dc.format.medium Electronic en
dc.language eng en
dc.relation.ispartofseries Pathogens (Basel, Switzerland) en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.rights.uri en
dc.title Impact of Superantigen-Producing Bacteria on T Cells from Tonsillar Hyperplasia. en
dc.type Journal Article en
dc.identifier.doi 10.3390/pathogens8030090 en
pubs.issue 3 en
pubs.volume 8 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.elements-id 775984 en Medical and Health Sciences en Medical Sciences en Molecular Medicine en School of Medicine en Surgery Department en Science en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 2076-0817 en
pubs.record-created-at-source-date 2019-06-30 en
pubs.dimensions-id 31252586 en

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