Impact of Superantigen-Producing Bacteria on T Cells from Tonsillar Hyperplasia.

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dc.contributor.author Radcliff, Fiona en
dc.contributor.author Waldvogel-Thurlow, Sharon en
dc.contributor.author Clow, Fiona en
dc.contributor.author Mahadevan, Murali en
dc.contributor.author Johnston, James en
dc.contributor.author Li, Gen en
dc.contributor.author Proft, Thomas en
dc.contributor.author Douglas, Richard en
dc.contributor.author Fraser, John en
dc.date.accessioned 2019-09-29T21:35:05Z en
dc.date.issued 2019-06-27 en
dc.identifier.citation Pathogens 8(3) 27 Jun 2019 en
dc.identifier.issn 2076-0817 en
dc.identifier.uri http://hdl.handle.net/2292/47969 en
dc.description.abstract Staphylococcus aureus and Group A Streptococcus (GAS) are common occupants of the tonsils and many strains produce potent exotoxins (mitogens) that directly target T cells, which could be a driver for tonsillar hyperplasia. Tonsil tissues from 41 patients were tested for these bacteria in conjunction with profiling of B and T cells by flow cytometry. S. aureus and GAS were detected in tonsil tissue from 44% and 7%, respectively, of patients by bacteriological culture; immuno-histology showed bacteria in close proximity to both B and T lymphocytes. The presence of tonsillar S. aureus did not alter B or T cell populations, whereas peripheral blood mucosal-associated invariant T (MAIT) cells were significantly increased in S. aureus culture positive individuals (p < 0.006). Alterations of tonsil CD4+ TCR Vβ family members relative to peripheral blood were evident in 29 patients. Three patients had strong TCR Vβ skewing indicative of recent exposure to superantigens, their tonsils contained mitogenic bacteria, and supernatants from these bacteria were used to partially recapitulate the skewing profile in vitro, supporting the notion that superantigens can target tonsillar T cells in situ. Tonsils are a reservoir for superantigen-producing bacteria with the capacity to alter the composition and function of key immune cells. en
dc.format.medium Electronic en
dc.language eng en
dc.relation.ispartofseries Pathogens (Basel, Switzerland) en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.title Impact of Superantigen-Producing Bacteria on T Cells from Tonsillar Hyperplasia. en
dc.type Journal Article en
dc.identifier.doi 10.3390/pathogens8030090 en
pubs.issue 3 en
pubs.volume 8 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.elements-id 775984 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Molecular Medicine en
pubs.org-id School of Medicine en
pubs.org-id Surgery Department en
pubs.org-id Science en
pubs.org-id Science Research en
pubs.org-id Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 2076-0817 en
pubs.record-created-at-source-date 2019-06-30 en
pubs.dimensions-id 31252586 en


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