A simple, economical and effective ELISA using simulated serum sample for teaching the concept and application of antigen–antibody interactions in undergraduate laboratory

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dc.contributor.author Khan, Latifa en
dc.contributor.author Tsai, Jia Yun en
dc.date.accessioned 2019-09-30T22:50:58Z en
dc.date.issued 2019 en
dc.identifier.issn 0021-9266 en
dc.identifier.uri http://hdl.handle.net/2292/48180 en
dc.description.abstract © 2019, © 2019 Royal Society of Biology. Enzyme-Linked Immunosorbent Assay (ELISA) is a versatile and widely used technique to detect and quantify small molecules in various clinical and analytical applications. It is also an effective tool for demonstrating the specificity between antibody and antigen to students across diverse disciplines. However, undergraduate laboratories often face the difficulty of performing proper ELISA exercise due to time and facility constraints. Here, we report a cost-effective, technically simple ELISA exercise which can be completed within 2.5-h at room temperature. This microtitre plate-based ELISA exercise utilises easily sourced inexpensive materials and simulated serum samples which are safe to handle in the undergraduate lab settings. The hands-on procedure and a real-life scenario of disease (Rheumatoid Arthritis) diagnosis reinforce students’ understanding of antigen–antibody interactions. ELISA results are displayed by a colourimetric enzymatic reaction, which can be analysed by eye without specialised equipment. This laboratory exercise was designed as part of the final year’s immunology course in a three-year undergraduate Biomedical Science programme, but can also be adapted to suit a broad range of student skill levels in science-fields because of its easy set-up and low technical requirement. The procedure and scenario can be also adjusted to meet the specific needs of other educator’s goals. en
dc.relation.ispartofseries Journal of Biological Education en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title A simple, economical and effective ELISA using simulated serum sample for teaching the concept and application of antigen–antibody interactions in undergraduate laboratory en
dc.type Journal Article en
dc.identifier.doi 10.1080/00219266.2019.1609552 en
pubs.volume online first en
dc.rights.holder Copyright: The author en
pubs.author-url https://www.tandfonline.com/doi/full/10.1080/00219266.2019.1609552 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 770381 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Molecular Medicine en
dc.identifier.eissn 2157-6009 en
pubs.record-created-at-source-date 2019-05-10 en
pubs.online-publication-date 2019-04-24 en


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