dc.contributor.author |
Delay, C |
en |
dc.contributor.author |
Chapman, K |
en |
dc.contributor.author |
Taleski, M |
en |
dc.contributor.author |
Wang, Y |
en |
dc.contributor.author |
Tyagi, S |
en |
dc.contributor.author |
Xiong, Y |
en |
dc.contributor.author |
Imin, Nijat |
en |
dc.contributor.author |
Djordjevic, MA |
en |
dc.date.accessioned |
2019-10-01T21:09:49Z |
en |
dc.date.issued |
2019-09 |
en |
dc.identifier.citation |
Journal of Experimental Botany 70(18):4763-4774 Sep 2019 |
en |
dc.identifier.issn |
0022-0957 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/48312 |
en |
dc.description.abstract |
CEPs (C-TERMINALLY ENCODED PEPTIDEs) inhibit Arabidopsis primary root growth by unknown mechanisms. We investigated how CEP3 levels control primary root growth. CEP3 peptide application decreased cell division, S-phase cell number, root meristematic cell number, and meristem zone (MZ) size in a dose- and CEP RECEPTOR1-dependent manner. Grafting showed that CEP3-dependent growth inhibition requires root and shoot CEPR1. CEP3 induced mitotic quiescence in MZ cells significantly faster than that induced by nutrient limitation alone. CEP3 also inhibited the restoration of S-phase to mitotically quiescence cells by nutrient resupply without quantitatively reducing TARGET OF RAPAMYCIN (TOR) kinase activity. In contrast, cep3-1 had an increased meristem size and S-phase cell number under nitrogen (N)-limited conditions, but not under N-sufficient conditions. Furthermore, cep3-1 meristematic cells remained in S-phase longer than wild-type cells during a sustained carbon (C) and N limitation. RNA sequencing showed that CEP3 peptide down-regulated genes involved in S-phase entry, cell wall and ribosome biogenesis, DNA replication, and meristem expansion, and up-regulated genes involved in catabolic processes and proteins and peptides that negatively control meristem expansion and root growth. Many of these genes were reciprocally regulated in cep3-1. The results suggest that raising CEP3 induces starvation-related responses that curtail primary root growth under severe nutrient limitation. |
en |
dc.publisher |
Oxford University Press (OUP) |
en |
dc.relation.ispartofseries |
Journal of Experimental Botany |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by-nc/4.0/ |
en |
dc.title |
CEP3 levels affect starvation-related growth responses of the primary root |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1093/jxb/erz270 |
en |
pubs.issue |
18 |
en |
pubs.begin-page |
4763 |
en |
pubs.volume |
70 |
en |
dc.rights.holder |
Copyright: The authors |
en |
pubs.end-page |
4774 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
774948 |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Biological Sciences |
en |
pubs.record-created-at-source-date |
2019-06-20 |
en |
pubs.dimensions-id |
31173100 |
en |