An approach to recombinantly produce mature grape polyphenol oxidase.

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dc.contributor.author Li, Yu en
dc.contributor.author McLarin, Mark-Anthony en
dc.contributor.author Middleditch, Martin en
dc.contributor.author Morrow, Stuart J en
dc.contributor.author Kilmartin, Paul en
dc.contributor.author Leung, Ka Ho Ivanhoe en
dc.date.accessioned 2019-10-02T00:42:01Z en
dc.date.issued 2019-10 en
dc.identifier.issn 0300-9084 en
dc.identifier.uri http://hdl.handle.net/2292/48332 en
dc.description.abstract Polyphenol oxidases (PPOs) are important enzymes that are widely found in both prokaryotes and eukaryotes including grapes. Studies of grape PPO to date have mostly relied on enzymes extracted and purified from plants. In this work, we describe the production of the mature form of Shine Muscat grape PPO by using an Escherichia coli expression system. We have optimised the purification procedure to obtain pure and active recombinant enzymes and characterised the catalytic efficiency of the recombinant grape PPO by using ultraviolet/visible (UV/Vis) spectrophotometry. Our work provides a simple protocol of obtaining pure and active recombinant grape PPO that will enable further studies about the catalytic mechanism and inhibition of this enzyme. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Biochimie en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Escherichia coli en
dc.subject Vitis en
dc.subject Catechol Oxidase en
dc.subject Plant Proteins en
dc.subject Recombinant Proteins en
dc.subject Cloning, Molecular en
dc.subject Kinetics en
dc.title An approach to recombinantly produce mature grape polyphenol oxidase. en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.biochi.2019.07.002 en
pubs.begin-page 40 en
pubs.volume 165 en
dc.rights.holder Copyright: The author en
pubs.end-page 47 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Journal Article en
pubs.elements-id 776622 en
pubs.org-id Science en
pubs.org-id Chemistry en
dc.identifier.eissn 1638-6183 en
pubs.record-created-at-source-date 2019-07-09 en
pubs.dimensions-id 31283975 en


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