Microfluidic affinity separation chip for selective capture and release of label-free ovarian cancer exosomes

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dc.contributor.author Hisey, Colin en
dc.contributor.author Dorayappan, KDP en
dc.contributor.author Cohn, DE en
dc.contributor.author Selvendiran, K en
dc.contributor.author Hansford, DJ en
dc.date.accessioned 2019-10-08T09:08:02Z en
dc.date.issued 2018-10-21 en
dc.identifier.issn 1473-0189 en
dc.identifier.uri http://hdl.handle.net/2292/48494 en
dc.description.abstract Exosomes are nanoscale vesicles found in many bodily fluids which play a significant role in cell-to-cell signaling and contain biomolecules indicative of their cells of origin. Recently, microfluidic devices have provided the ability to efficiently capture exosomes based on specific membrane biomarkers, but releasing the captured exosomes intact and label-free for downstream characterization and experimentation remains a challenge. We present a herringbone-grooved microfluidic device which is covalently functionalized with antibodies against general and cancer exosome membrane biomarkers (CD9 and EpCAM) to isolate exosomes from small volumes of high-grade serous ovarian cancer (HGSOC) serum. Following capture, intact exosomes are released label-free using a low pH buffer and immediately neutralized downstream to ensure their stability. Characterization of captured and released exosomes was performed using fluorescence microscopy, nanoparticle tracking analysis, flow-cytometry, and SEM. Our results demonstrate the successful isolation of intact and label-free exosomes, indicate that the amount of both total and EpCAM+ exosomes increases with HGSOC disease progression, and demonstrate the ownstream internalization of isolated exosomes by OVCAR8 cells. This device and approach can be utilized for a nearly limitless range of downstream exosome analytical and experimental techniques, both on and off-chip. en
dc.publisher Royal Society of Chemistry en
dc.relation.ispartofseries Lab on a Chip en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Microfluidic affinity separation chip for selective capture and release of label-free ovarian cancer exosomes en
dc.type Journal Article en
dc.identifier.doi 10.1039/c8lc00834e en
pubs.issue 20 en
pubs.begin-page 3144 en
pubs.volume 18 en
dc.rights.holder Copyright: The author en
pubs.end-page 3153 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 776145 en
pubs.org-id Medical and Health Sciences en
pubs.org-id School of Medicine en
pubs.org-id Obstetrics and Gynaecology en
pubs.record-created-at-source-date 2019-07-08 en
pubs.online-publication-date 2018-09-04 en

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