dc.contributor.author |
Hisey, Colin |
en |
dc.contributor.author |
Dorayappan, KDP |
en |
dc.contributor.author |
Cohn, DE |
en |
dc.contributor.author |
Selvendiran, K |
en |
dc.contributor.author |
Hansford, DJ |
en |
dc.date.accessioned |
2019-10-08T09:08:02Z |
en |
dc.date.issued |
2018-10-21 |
en |
dc.identifier.issn |
1473-0189 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/48494 |
en |
dc.description.abstract |
Exosomes are nanoscale vesicles found in many bodily fluids which play a significant role in cell-to-cell signaling and contain biomolecules indicative of their cells of origin. Recently, microfluidic devices have provided the ability to efficiently capture exosomes based on specific membrane biomarkers, but releasing the captured exosomes intact and label-free for downstream characterization and experimentation remains a challenge. We present a herringbone-grooved microfluidic device which is covalently functionalized with antibodies against general and cancer exosome membrane biomarkers (CD9 and EpCAM) to isolate exosomes from small volumes of high-grade serous ovarian cancer (HGSOC) serum. Following capture, intact exosomes are released label-free using a low pH buffer and immediately neutralized downstream to ensure their stability. Characterization of captured and released exosomes was performed using fluorescence microscopy, nanoparticle tracking analysis, flow-cytometry, and SEM. Our results demonstrate the successful isolation of intact and label-free exosomes, indicate that the amount of both total and EpCAM+ exosomes increases with HGSOC disease progression, and demonstrate the ownstream internalization of isolated exosomes by OVCAR8 cells. This device and approach can be utilized for a nearly limitless range of downstream exosome analytical and experimental techniques, both on and off-chip. |
en |
dc.publisher |
Royal Society of Chemistry |
en |
dc.relation.ispartofseries |
Lab on a Chip |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Microfluidic affinity separation chip for selective capture and release of label-free ovarian cancer exosomes |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1039/c8lc00834e |
en |
pubs.issue |
20 |
en |
pubs.begin-page |
3144 |
en |
pubs.volume |
18 |
en |
dc.rights.holder |
Copyright: The author |
en |
pubs.end-page |
3153 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
776145 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Obstetrics and Gynaecology |
en |
pubs.record-created-at-source-date |
2019-07-08 |
en |
pubs.online-publication-date |
2018-09-04 |
en |