Effect of consecutive rare codons on the recombinant production of human proteins in Escherichia coli.

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dc.contributor.author Correddu, Danilo en
dc.contributor.author Montaño López, José de Jesús en
dc.contributor.author Angermayr, S Andreas en
dc.contributor.author Middleditch, Martin en
dc.contributor.author Payne, Leo S en
dc.contributor.author Leung, Ka Ho Ivanhoe en
dc.date.accessioned 2019-10-29T02:38:56Z en
dc.date.issued 2020-02 en
dc.identifier.issn 1521-6543 en
dc.identifier.uri http://hdl.handle.net/2292/48731 en
dc.description.abstract In Escherichia coli, the expression of heterologous genes for the production of recombinant proteins can be challenging due to the codon bias of different organisms. The rare codons AGG and AGA are among the rarest in E. coli. In this work, by using the human gene RioK2 as case study, we found that the presence of consecutive AGG-AGA led to a premature stop, which may be caused by an event of -1 frameshift. We found that translational problems caused by consecutive AGG-AGA are sequence dependent, in particular, in sequences that contain multiple rare AGG or AGA codons elsewhere. Translational problems can be alleviated by different strategies, including codon harmonization, codon optimization, or by substituting the consecutive AGG-AGA codons by more frequent arginine codons. Overall, our results furthered our understanding about the relationship between consecutive rare codons and translational problems. Such information will aid the design of DNA sequence for the production of recombinant proteins. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries IUBMB life en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Effect of consecutive rare codons on the recombinant production of human proteins in Escherichia coli. en
dc.type Journal Article en
dc.identifier.doi 10.1002/iub.2162 en
pubs.issue 2 en
pubs.begin-page 266 en
pubs.volume 72 en
dc.rights.holder Copyright: The author en
pubs.end-page 274 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype Journal Article en
pubs.elements-id 780795 en
pubs.org-id Science en
pubs.org-id Chemistry en
dc.identifier.eissn 1521-6551 en
pubs.record-created-at-source-date 2019-09-12 en
pubs.dimensions-id 31509345 en

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