dc.contributor.author |
Torres Cleuren, Yamila N |
en |
dc.contributor.author |
Ewe, Chee Kiang |
en |
dc.contributor.author |
Chipman, Kyle C |
en |
dc.contributor.author |
Mears, Emily |
en |
dc.contributor.author |
Wood, Cricket G |
en |
dc.contributor.author |
Al-Alami, Coco Emma Alma |
en |
dc.contributor.author |
Alcorn, Melissa R |
en |
dc.contributor.author |
Turner, Thomas L |
en |
dc.contributor.author |
Joshi, Pradeep M |
en |
dc.contributor.author |
Snell, Russell |
en |
dc.contributor.author |
Rothman, Joel H |
en |
dc.date.accessioned |
2019-11-03T21:46:00Z |
en |
dc.date.issued |
2019-08-15 |
en |
dc.identifier.citation |
eLife 8 15 Aug 2019 |
en |
dc.identifier.issn |
2050-084X |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/48804 |
en |
dc.description.abstract |
Innovations in metazoan development arise from evolutionary modification of gene regulatory networks (GRNs). We report widespread cryptic variation in the requirement for two key regulatory inputs, SKN-1/Nrf2 and MOM-2/Wnt, into the C. elegans endoderm GRN. While some natural isolates show a nearly absolute requirement for these two regulators, in others, most embryos differentiate endoderm in their absence. GWAS and analysis of recombinant inbred lines reveal multiple genetic regions underlying this broad phenotypic variation. We observe a reciprocal trend, in which genomic variants, or knockdown of endoderm regulatory genes, that result in a high SKN-1 requirement often show low MOM-2/Wnt requirement and vice-versa, suggesting that cryptic variation in the endoderm GRN may be tuned by opposing requirements for these two key regulatory inputs. These findings reveal that while the downstream components in the endoderm GRN are common across metazoan phylogeny, initiating regulatory inputs are remarkably plastic even within a single species. |
en |
dc.format.medium |
Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
eLife |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
en |
dc.subject |
Animals |
en |
dc.subject |
Caenorhabditis elegans |
en |
dc.subject |
Intracellular Signaling Peptides and Proteins |
en |
dc.subject |
DNA-Binding Proteins |
en |
dc.subject |
Caenorhabditis elegans Proteins |
en |
dc.subject |
Transcription Factors |
en |
dc.subject |
Gene Expression Regulation, Developmental |
en |
dc.subject |
Wnt Proteins |
en |
dc.subject |
Gene Regulatory Networks |
en |
dc.subject |
Genetic Variation |
en |
dc.subject |
Progranulins |
en |
dc.title |
Extensive intraspecies cryptic variation in an ancient embryonic gene regulatory network. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.7554/elife.48220 |
en |
pubs.volume |
8 |
en |
dc.rights.holder |
Copyright: The authors |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
research-article |
en |
pubs.subtype |
Journal Article |
en |
pubs.subtype |
Research Support, N.I.H., Extramural |
en |
pubs.elements-id |
783904 |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Biological Sciences |
en |
dc.identifier.eissn |
2050-084X |
en |
pubs.record-created-at-source-date |
2019-08-16 |
en |
pubs.dimensions-id |
31414984 |
en |