Extensive intraspecies cryptic variation in an ancient embryonic gene regulatory network.

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dc.contributor.author Torres Cleuren, Yamila N en
dc.contributor.author Ewe, Chee Kiang en
dc.contributor.author Chipman, Kyle C en
dc.contributor.author Mears, Emily en
dc.contributor.author Wood, Cricket G en
dc.contributor.author Al-Alami, Coco Emma Alma en
dc.contributor.author Alcorn, Melissa R en
dc.contributor.author Turner, Thomas L en
dc.contributor.author Joshi, Pradeep M en
dc.contributor.author Snell, Russell en
dc.contributor.author Rothman, Joel H en
dc.date.accessioned 2019-11-03T21:46:00Z en
dc.date.issued 2019-08-15 en
dc.identifier.citation eLife 8 15 Aug 2019 en
dc.identifier.issn 2050-084X en
dc.identifier.uri http://hdl.handle.net/2292/48804 en
dc.description.abstract Innovations in metazoan development arise from evolutionary modification of gene regulatory networks (GRNs). We report widespread cryptic variation in the requirement for two key regulatory inputs, SKN-1/Nrf2 and MOM-2/Wnt, into the C. elegans endoderm GRN. While some natural isolates show a nearly absolute requirement for these two regulators, in others, most embryos differentiate endoderm in their absence. GWAS and analysis of recombinant inbred lines reveal multiple genetic regions underlying this broad phenotypic variation. We observe a reciprocal trend, in which genomic variants, or knockdown of endoderm regulatory genes, that result in a high SKN-1 requirement often show low MOM-2/Wnt requirement and vice-versa, suggesting that cryptic variation in the endoderm GRN may be tuned by opposing requirements for these two key regulatory inputs. These findings reveal that while the downstream components in the endoderm GRN are common across metazoan phylogeny, initiating regulatory inputs are remarkably plastic even within a single species. en
dc.format.medium Electronic en
dc.language eng en
dc.relation.ispartofseries eLife en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Animals en
dc.subject Caenorhabditis elegans en
dc.subject Intracellular Signaling Peptides and Proteins en
dc.subject DNA-Binding Proteins en
dc.subject Caenorhabditis elegans Proteins en
dc.subject Transcription Factors en
dc.subject Gene Expression Regulation, Developmental en
dc.subject Wnt Proteins en
dc.subject Gene Regulatory Networks en
dc.subject Genetic Variation en
dc.subject Progranulins en
dc.title Extensive intraspecies cryptic variation in an ancient embryonic gene regulatory network. en
dc.type Journal Article en
dc.identifier.doi 10.7554/elife.48220 en
pubs.volume 8 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.subtype Research Support, N.I.H., Extramural en
pubs.elements-id 783904 en
pubs.org-id Science en
pubs.org-id Biological Sciences en
dc.identifier.eissn 2050-084X en
pubs.record-created-at-source-date 2019-08-16 en
pubs.dimensions-id 31414984 en


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