Evaluation of parylene derivatives for use as biomaterials for human astrocyte cell patterning.

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dc.contributor.author Raos, Brad en
dc.contributor.author Simpson, M Cather en
dc.contributor.author Doyle, Colin S en
dc.contributor.author Graham, Euan en
dc.contributor.author Unsworth, Charles en
dc.date.accessioned 2019-11-04T02:05:36Z en
dc.date.issued 2019-01 en
dc.identifier.citation PloS one 14(6):e0218850 Jan 2019 en
dc.identifier.issn 1932-6203 en
dc.identifier.uri http://hdl.handle.net/2292/48811 en
dc.description.abstract Cell patterning is becoming increasingly popular in neuroscience because it allows for the control in the location and connectivity of cells. A recently developed cell patterning technology uses patterns of an organic polymer, parylene-C, on a background of SiO2. When cells are cultured on the parylene-C/SiO2 substrate they conform to the underlying parylene-C geometry. Parylene-C is, however, just one member of a family of parylene polymers that have varying chemical and physical properties. In this work, we investigate whether two commercially available mainstream parylene derivatives, parylene-D, parylene-N and a more recent parylene derivative, parylene-HT to determine if they enable higher fidelity hNT astrocyte cell patterning compared to parylene-C. We demonstrate that all parylene derivatives are compatible with the existing laser fabrication method. We then demonstrate that parylene-HT, parylene-D and parylene-N are suitable for use as an hNT astrocyte cell attractive substrate and result in an equal quality of patterning compared to parylene-C. This work supports the use of alternative parylene derivatives for applications where their different physical and chemical properties are more suitable. en
dc.format.medium Electronic-eCollection en
dc.language eng en
dc.relation.ispartofseries PloS one en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Nerve Net en
dc.subject Astrocytes en
dc.subject Cell Line en
dc.subject Humans en
dc.subject Silicon Dioxide en
dc.subject Xylenes en
dc.subject Polymers en
dc.subject Biocompatible Materials en
dc.subject Cell Culture Techniques en
dc.subject Materials Testing en
dc.subject Cell Differentiation en
dc.subject Calcium Signaling en
dc.subject Surface Properties en
dc.title Evaluation of parylene derivatives for use as biomaterials for human astrocyte cell patterning. en
dc.type Journal Article en
dc.identifier.doi 10.1371/journal.pone.0218850 en
pubs.issue 6 en
pubs.begin-page e0218850 en
pubs.volume 14 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.elements-id 777486 en
pubs.org-id Engineering en
pubs.org-id Engineering Science en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Molecular Medicine en
pubs.org-id Pharmacy en
dc.identifier.eissn 1932-6203 en
pubs.record-created-at-source-date 2019-06-26 en
pubs.dimensions-id 31237927 en


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