dc.contributor.author |
Raos, Brad |
en |
dc.contributor.author |
Simpson, M Cather |
en |
dc.contributor.author |
Doyle, Colin S |
en |
dc.contributor.author |
Graham, Euan |
en |
dc.contributor.author |
Unsworth, Charles |
en |
dc.date.accessioned |
2019-11-04T02:05:36Z |
en |
dc.date.issued |
2019-01 |
en |
dc.identifier.citation |
PloS one 14(6):e0218850 Jan 2019 |
en |
dc.identifier.issn |
1932-6203 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/48811 |
en |
dc.description.abstract |
Cell patterning is becoming increasingly popular in neuroscience because it allows for the control in the location and connectivity of cells. A recently developed cell patterning technology uses patterns of an organic polymer, parylene-C, on a background of SiO2. When cells are cultured on the parylene-C/SiO2 substrate they conform to the underlying parylene-C geometry. Parylene-C is, however, just one member of a family of parylene polymers that have varying chemical and physical properties. In this work, we investigate whether two commercially available mainstream parylene derivatives, parylene-D, parylene-N and a more recent parylene derivative, parylene-HT to determine if they enable higher fidelity hNT astrocyte cell patterning compared to parylene-C. We demonstrate that all parylene derivatives are compatible with the existing laser fabrication method. We then demonstrate that parylene-HT, parylene-D and parylene-N are suitable for use as an hNT astrocyte cell attractive substrate and result in an equal quality of patterning compared to parylene-C. This work supports the use of alternative parylene derivatives for applications where their different physical and chemical properties are more suitable. |
en |
dc.format.medium |
Electronic-eCollection |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
PloS one |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
en |
dc.subject |
Nerve Net |
en |
dc.subject |
Astrocytes |
en |
dc.subject |
Cell Line |
en |
dc.subject |
Humans |
en |
dc.subject |
Silicon Dioxide |
en |
dc.subject |
Xylenes |
en |
dc.subject |
Polymers |
en |
dc.subject |
Biocompatible Materials |
en |
dc.subject |
Cell Culture Techniques |
en |
dc.subject |
Materials Testing |
en |
dc.subject |
Cell Differentiation |
en |
dc.subject |
Calcium Signaling |
en |
dc.subject |
Surface Properties |
en |
dc.title |
Evaluation of parylene derivatives for use as biomaterials for human astrocyte cell patterning. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1371/journal.pone.0218850 |
en |
pubs.issue |
6 |
en |
pubs.begin-page |
e0218850 |
en |
pubs.volume |
14 |
en |
dc.rights.holder |
Copyright: The authors |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
en |
pubs.subtype |
research-article |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
777486 |
en |
pubs.org-id |
Engineering |
en |
pubs.org-id |
Engineering Science |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
Pharmacy |
en |
dc.identifier.eissn |
1932-6203 |
en |
pubs.record-created-at-source-date |
2019-06-26 |
en |
pubs.dimensions-id |
31237927 |
en |