Make it simple: long-term stable gradient generation in a microfluidic microdevice.

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Show simple item record Parittotokkaporn, Sam en Dravid, Anusha en Bansal, Mahima en Aqrawe, Zaid en Svirskis, Darren en Suresh, Vinod en OCarroll, Simon en 2019-11-04T19:38:53Z en 2019-07-26 en
dc.identifier.issn 1387-2176 en
dc.identifier.uri en
dc.description.abstract Microfluidics-based gradient generators have been used for various biological applications, specifically chemotaxis in cell culture. However, the ability to generate and maintain long term gradients alongside the ability to quickly switch solutions is a challenge of the current microfabricated systems. In this study, a simple flow-driven microfluidic system was developed to achieve long-term stable concentration gradients. Computational modelling was performed to highlight the fluid dynamics as well as to verify the ability of maintaining stable gradients over 7 days. Numerical simulation was analysed to evaluate the static pressure, velocity magnitude and wall shear stress distribution in the chamber. A microdevice fabricated with polydimethylsiloxane (PDMS), using a standard soft lithography technique is presented. It consists of eight parallel microchannels (5 μm × 30 μm × 1,800 μm) linking source and sink chambers; a syringe pump drives fluid through the sink chamber, advection/diffusion from source to sink establishes a gradient. A gradient of a fluorescent dye was generated under the low flow control at 1-10 μl/h of a simple syringe pump equipped with a pulsation damper that was comparable to a pulseless microfluidic pump. Concentration gradients were formed in 1 h and stable from 2 h out to 5 days and consuming less than 1.0 ml of solution. This study focuses on a novel solution to achieve a long-term microfluidic gradient generator using simple engineering techniques of biomedical microdevices. en
dc.format.medium Electronic en
dc.language eng en
dc.relation.ispartofseries Biomedical microdevices en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.title Make it simple: long-term stable gradient generation in a microfluidic microdevice. en
dc.type Journal Article en
dc.identifier.doi 10.1007/s10544-019-0427-4 en
pubs.issue 3 en
pubs.begin-page 77 en
pubs.volume 21 en
dc.rights.holder Copyright: The author en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype Journal Article en
pubs.elements-id 778430 en Bioengineering Institute en ABI Associates en Engineering en Engineering Science en Medical and Health Sciences en Medical Sciences en Anatomy and Medical Imaging en Pharmacy en Science en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1572-8781 en
pubs.record-created-at-source-date 2019-07-27 en
pubs.dimensions-id 31346791 en

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