Make it simple: long-term stable gradient generation in a microfluidic microdevice.

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dc.contributor.author Parittotokkaporn, Sam en
dc.contributor.author Dravid, Anusha en
dc.contributor.author Bansal, Mahima en
dc.contributor.author Aqrawe, Zaid en
dc.contributor.author Svirskis, Darren en
dc.contributor.author Suresh, Vinod en
dc.contributor.author OCarroll, Simon en
dc.date.accessioned 2019-11-04T19:38:53Z en
dc.date.issued 2019-07-26 en
dc.identifier.issn 1387-2176 en
dc.identifier.uri http://hdl.handle.net/2292/48825 en
dc.description.abstract Microfluidics-based gradient generators have been used for various biological applications, specifically chemotaxis in cell culture. However, the ability to generate and maintain long term gradients alongside the ability to quickly switch solutions is a challenge of the current microfabricated systems. In this study, a simple flow-driven microfluidic system was developed to achieve long-term stable concentration gradients. Computational modelling was performed to highlight the fluid dynamics as well as to verify the ability of maintaining stable gradients over 7 days. Numerical simulation was analysed to evaluate the static pressure, velocity magnitude and wall shear stress distribution in the chamber. A microdevice fabricated with polydimethylsiloxane (PDMS), using a standard soft lithography technique is presented. It consists of eight parallel microchannels (5 μm × 30 μm × 1,800 μm) linking source and sink chambers; a syringe pump drives fluid through the sink chamber, advection/diffusion from source to sink establishes a gradient. A gradient of a fluorescent dye was generated under the low flow control at 1-10 μl/h of a simple syringe pump equipped with a pulsation damper that was comparable to a pulseless microfluidic pump. Concentration gradients were formed in 1 h and stable from 2 h out to 5 days and consuming less than 1.0 ml of solution. This study focuses on a novel solution to achieve a long-term microfluidic gradient generator using simple engineering techniques of biomedical microdevices. en
dc.format.medium Electronic en
dc.language eng en
dc.relation.ispartofseries Biomedical microdevices en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Make it simple: long-term stable gradient generation in a microfluidic microdevice. en
dc.type Journal Article en
dc.identifier.doi 10.1007/s10544-019-0427-4 en
pubs.issue 3 en
pubs.begin-page 77 en
pubs.volume 21 en
dc.rights.holder Copyright: The author en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype Journal Article en
pubs.elements-id 778430 en
pubs.org-id Bioengineering Institute en
pubs.org-id ABI Associates en
pubs.org-id Engineering en
pubs.org-id Engineering Science en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Anatomy and Medical Imaging en
pubs.org-id Pharmacy en
pubs.org-id Science en
pubs.org-id Science Research en
pubs.org-id Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1572-8781 en
pubs.record-created-at-source-date 2019-07-27 en
pubs.dimensions-id 31346791 en


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