GABAA Receptors Are Well Preserved in the Hippocampus of Aged Mice.

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dc.contributor.author Palpagama, Thulani H en
dc.contributor.author Sagniez, Mélanie en
dc.contributor.author Kim, SooHyun en
dc.contributor.author Waldvogel, Henry en
dc.contributor.author Faull, Richard en
dc.contributor.author Kwakowsky, Andrea en
dc.date.accessioned 2019-11-25T01:18:53Z en
dc.date.issued 2019-07 en
dc.identifier.citation eNeuro 6(4) Jul 2019 en
dc.identifier.issn 2373-2822 en
dc.identifier.uri http://hdl.handle.net/2292/49147 en
dc.description.abstract GABA is the primary inhibitory neurotransmitter in the nervous system. GABAA receptors (GABAARs) are pentameric ionotropic channels. Subunit composition of the receptors is associated with the affinity of GABA binding and its downstream inhibitory actions. Fluctuations in subunit expression levels with increasing age have been demonstrated in animal and human studies. However, our knowledge regarding the age-related hippocampal GABAAR expression changes is limited and based on rat studies. This study is the first analysis of the aging-related changes of the GABAAR subunit expression in the CA1, CA2/3, and dentate gyrus regions of the mouse hippocampus. Using Western blotting and immunohistochemistry we found that the GABAergic system is robust, with no significant age-related differences in GABAAR α1, α2, α3, α5, β3, and γ2 subunit expression level differences found between the young (6 months) and old (21 months) age groups in any of the hippocampal regions examined. However, we detected a localized decrease of α2 subunit expression around the soma, proximal dendrites, and in the axon initial segment of pyramidal cells in the CA1 and CA3 regions that is accompanied by a pronounced upregulation of the α2 subunit immunoreactivity in the neuropil of aged mice. In summary, GABAARs are well preserved in the mouse hippocampus during normal aging although GABAARs in the hippocampus are severely affected in age-related neurological disorders, including Alzheimer's disease. en
dc.format.medium Electronic-Print en
dc.language eng en
dc.relation.ispartofseries eNeuro en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Hippocampus en
dc.subject Pyramidal Cells en
dc.subject Animals en
dc.subject Mice, Inbred C57BL en
dc.subject Receptors, GABA-A en
dc.subject Protein Subunits en
dc.subject Aging en
dc.subject Male en
dc.subject Axon Initial Segment en
dc.title GABAA Receptors Are Well Preserved in the Hippocampus of Aged Mice. en
dc.type Journal Article en
dc.identifier.doi 10.1523/eneuro.0496-18.2019 en
pubs.issue 4 en
pubs.volume 6 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.elements-id 778509 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Anatomy and Medical Imaging en
dc.identifier.eissn 2373-2822 en
pubs.record-created-at-source-date 2019-07-26 en
pubs.dimensions-id 31340951 en


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