Identification and characterisation of the Bcvic1 and Bcvic2 vegetative incompatibility genes in Botrytis cinerea

Show simple item record

dc.contributor.advisor Templeton, Matt en
dc.contributor.advisor Pearson, Mike en Arshed, Saadiah en 2019-11-25T01:44:24Z en 2019 en
dc.identifier.uri en
dc.description.abstract Vegetative incompatibility (VI) is a fungal non-self recognition system characterised by the inability of genetically distinct conspecific fungal strains to form a viable heterokaryon, and is controlled by multiple polymorphic loci termed vic (vegetative incompatibility) or het (heterokaryon incompatibility). In this thesis, I report the first vic locus that has been genetically identified and characterised in the economically important plant pathogenic, necrotrophic fungus Botrytis cinerea. A bulk segregant approach coupled with whole genome Illumina sequencing was used to map a 60-kb genomic region for the vic locus in near isogenic lines of interacting compatible and incompatible strains of B. cinerea. Within that locus, I identified two adjacent highly polymorphic open reading frames encoding predicted proteins that contain domain architectures implicated in VI in other filamentous fungi, Bcvic1 and Bcvic2. Bcvic1 encodes a predicted protein containing the NACHT family of NTPases, Ankyrin repeats and a putative serine esterase. Bcvic2 encodes a putative syntaxin protein containing a SNARE domain, which functions in vesicular transport. Deletion of Bcvic1 and Bcvic2 individually did not have any effect on vegetative incompatibility. However, deletion of the region containing both Bcvic1 and Bcvic2 resulted in gene deletion lines that were severely restricted in growth and also showed loss of VI. Complementation of these mutants by ectopic expression restored the growth and vegetative incompatibility phenotype indicating that Bcvic1 and Bcvic2 are interacting and controlling VI at this vic locus.
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof PhD Thesis - University of Auckland en
dc.relation.isreferencedby UoA99265326613902091 en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.rights.uri en
dc.title Identification and characterisation of the Bcvic1 and Bcvic2 vegetative incompatibility genes in Botrytis cinerea en
dc.type Thesis en Biological Sciences en The University of Auckland en Doctoral en PhD en
dc.rights.holder Copyright: The author en
dc.rights.accessrights en
pubs.elements-id 786740 en
pubs.record-created-at-source-date 2019-11-25 en
dc.identifier.wikidata Q112552495

Files in this item

Find Full text

This item appears in the following Collection(s)

Show simple item record


Search ResearchSpace