dc.contributor.advisor |
Quek, S |
en |
dc.contributor.advisor |
Bishop, K |
en |
dc.contributor.advisor |
Ferguson, LR |
en |
dc.contributor.author |
Peng, Yaoyao |
en |
dc.date.accessioned |
2019-11-26T01:45:26Z |
en |
dc.date.issued |
2019 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/49211 |
en |
dc.description.abstract |
Feijoa (feijoa sellowiana) is a subtropical fruit belonging to the myrtle family. The
species was discovered in South America in the late 1800s and is now widely grown in
New Zealand. In recent years, the fruit is becoming increasingly popular worldwide, and
the unique aroma and potential bioactivities are two major characteristics of the fruit.
However, to date, there is limited research on the characterization of the aroma-active
and bioactive compounds, and the potential health benefits of feijoa fruits.
Four New Zealand grown feijoa cultivars were utilized in this research. The aroma-active
compounds in feijoa flesh, peel, whole fruit, juice and essential oil were characterized by
HS-SPME-GC-O-MS. Bioactive compounds were extracted from feijoa flesh, peel and
whole fruit using optimized extraction methods by orthogonal design. The phenolic
profiles of each feijoa extract and feijoa juice were established by an optimized LC-ESIMS/
MS method. Thereafter, antioxidant activity was assessed by DPPH and FRAP
assays. The potential anti-inflammatory activity of feijoa extracts was investigated on
genetically modified cell models, namely HEK-Blue™ hTLR2, HEK-Blue™ hTLR4,
NOD2-WT and NOD2-G908R. Potential anti-cancer activity was explored using human
prostate cancer cell lines including LNCaP, DU145 and PC3.
Aroma profiles of each feijoa sample were successfully established according to the
perceived aroma active compounds and their aroma intensity. A total of seven
compounds in the phenolic profile of feijoa extracts, were reported for the first time in
feijoa fruits. The anti-inflammatory activity of feijoa flesh was detected only through the
TLR2 pathway, and the activity of feijoa peel and whole fruit was evident mainly through
the TLR2 and NOD2 pathways. Moreover, all feijoa extracts showed promising anticancer
activity in the LNCaP cell line through apoptosis pathways.
This study was the first systematic report on the characterization of aroma active and
phenolic compounds, and bioactivities of feijoa fruits. This confirmed the great potential
of feijoa fruits to be applied in food flavouring, functional foods, and pharmaceutical
industries. Furthermore, the research also provided scientific evidence on cultivar
selection of feijoa fruits to local growers and industries. |
|
dc.publisher |
ResearchSpace@Auckland |
en |
dc.relation.ispartof |
PhD Thesis - University of Auckland |
en |
dc.relation.isreferencedby |
UoA99265208013902091 |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights |
Restricted Item. Thesis embargoed until 11/2021. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
http://creativecommons.org/licenses/by-nc-nd/3.0/nz/ |
en |
dc.title |
Compositional characterization and potential health benefits of New Zealand grown feijoa cultivars |
en |
dc.type |
Thesis |
en |
thesis.degree.discipline |
Chemistry |
en |
thesis.degree.grantor |
The University of Auckland |
en |
thesis.degree.level |
Doctoral |
en |
thesis.degree.name |
PhD |
en |
dc.rights.holder |
Copyright: The author |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.elements-id |
786850 |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Chemistry |
en |
pubs.record-created-at-source-date |
2019-11-26 |
en |