Mechanical loading of isolated cardiac muscle with a real-time computed Windkessel model of the vasculature impedance.

Show simple item record Garrett, Amy S en Pham, Toan en Loiselle, Denis en Han, June en Taberner, Andrew en 2019-11-26T02:41:07Z en 2019-09 en
dc.identifier.citation Physiological Reports 7(17):e14184 Sep 2019 en
dc.identifier.issn 2051-817X en
dc.identifier.uri en
dc.description.abstract To date, the mechanical loads imposed on isolated cardiac muscle tissue in vitro have been oversimplified. Researchers typically applied loads that are time-invariant, resulting in either isometric and auxotonic contractions, or flat-topped (isotonic shortening) work-loops. These contraction types do not fully capture the dynamic response of contracting tissues adapting to a variable load, such as is experienced by ventricular tissue in vivo. In this study, we have successfully developed a loading system that presents a model-based, time-varying, continuously updated, load to cardiac tissue preparations. We combined a Windkessel model of vascular fluid impedance together with Laplace's Law and encoded it in a real-time hardware-based force-length control system. Experiments were carried out on isolated rat left ventricular trabeculae; we directly compare the work-loops arising from this protocol with those of a typical simplified isotonic shortening work-loop system. We found that, under body conditions, cardiac trabeculae achieved greater mechanical work output against our new loading system, than with the simplified isotonic work-loop protocol. We further tested whether loading the tissue with a mechanical impedance defined by "diseased" Windkessel model parameters had an effect on the performance of healthy trabeculae. We found that trabecula shortening decreased when applying the set of Windkessel parameters describing the hypertensive condition, and increased in the hypotensive state. Our implementation of a real-time model of arterial characteristics provides an improved, physiologically derived, instantly calculated load for use in studying isolated cardiac muscle, and is readily applicable to study various disease conditions. en
dc.format.medium Print en
dc.language eng en
dc.relation.ispartofseries Physiological reports en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.rights.uri en
dc.title Mechanical loading of isolated cardiac muscle with a real-time computed Windkessel model of the vasculature impedance. en
dc.type Journal Article en
dc.identifier.doi 10.14814/phy2.14184 en
pubs.issue 17 en
pubs.begin-page e14184 en
pubs.volume 7 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.elements-id 782687 en Bioengineering Institute en ABI Associates en Medical and Health Sciences en Medical Sciences en Nutrition en
dc.identifier.eissn 2051-817X en
pubs.record-created-at-source-date 2019-09-13 en
pubs.dimensions-id 31512409 en

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