dc.contributor.author |
Raj, Ashok |
en |
dc.contributor.author |
Shanahan, ER |
en |
dc.contributor.author |
Tran, CD |
en |
dc.contributor.author |
Bhat, P |
en |
dc.contributor.author |
Fletcher, LM |
en |
dc.contributor.author |
Vesey, DA |
en |
dc.contributor.author |
Morrison, M |
en |
dc.contributor.author |
Holtmann, G |
en |
dc.contributor.author |
MacDonald, GA |
en |
dc.date.accessioned |
2020-01-09T00:29:25Z |
en |
dc.date.issued |
2019-08-01 |
en |
dc.identifier.citation |
Clinical and Translational Gastroenterology 10(8) Article number e00068 Aug 2019 |
en |
dc.identifier.issn |
2155-384X |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/49427 |
en |
dc.description.abstract |
OBJECTIVES: Chronic liver disease (CLD) is associated with both alterations of the stool microbiota and increased small intestinal permeability. However, little is known about the role of the small intestinal mucosa-associated microbiota (MAM) in CLD. The aim of this study was to evaluate the relationship between the duodenal MAM and both small intestinal permeability and liver disease severity in CLD. METHODS: Subjects with CLD and a disease-free control group undergoing routine endoscopy underwent duodenal biopsy to assess duodenal MAM by 16S rRNA gene sequencing. Small intestinal permeability was assessed by a dual sugar (lactulose: rhamnose) assay. Other assessments included transient elastography, endotoxemia, serum markers of hepatic inflammation, dietary intake, and anthropometric measurements. RESULTS: Forty-six subjects (35 with CLD and 11 controls) were assessed. In subjects with CLD, the composition (P = 0.02) and diversity (P < 0.01) of the duodenal MAM differed to controls. Constrained multivariate analysis and linear discriminate effect size showed this was due to Streptococcus-affiliated lineages. Small intestinal permeability was significantly higher in CLD subjects compared to controls. In CLD, there were inverse correlations between microbial diversity and both increased small intestinal permeability (r = −0.41, P = 0.02) and serum alanine aminotransferase (r = −0.35, P = 0.04). Hepatic stiffness was not associated with the MAM. DISCUSSION: In CLD, there is dysbiosis of the duodenal MAM and an inverse correlation between microbial diversity and small intestinal permeability. TRANSLATIONAL IMPACT: Strategies to ameliorate duodenal MAM dysbiosis may ameliorate intestinal barrier dysfunction and liver injury in CLD. |
en |
dc.publisher |
Nature Publishing Group |
en |
dc.relation.ispartofseries |
Clinical and Translational Gastroenterology |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
en |
dc.title |
Dysbiosis of the Duodenal Mucosal Microbiota Is Associated With Increased Small Intestinal Permeability in Chronic Liver Disease |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.14309/ctg.0000000000000068 |
en |
pubs.issue |
8 |
en |
pubs.volume |
10 |
en |
dc.rights.holder |
Copyright: The authors |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
779865 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Medicine Department |
en |
pubs.number |
e00068 |
en |
pubs.record-created-at-source-date |
2019-09-01 |
en |
pubs.dimensions-id |
31373933 |
en |