Feasibility of using a luminescence-based method to determine serum bactericidal activity against Neisseria gonorrhoeae

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dc.contributor.author Clow, F en
dc.contributor.author O'Hanlon, Conor en
dc.contributor.author Christodoulides, M en
dc.contributor.author Radcliff, Fiona en
dc.date.accessioned 2020-02-11T22:08:26Z en
dc.date.issued 2019-12-01 en
dc.identifier.citation Vaccines 7(4) 01 Dec 2019 en
dc.identifier.issn 2076-393X en
dc.identifier.uri http://hdl.handle.net/2292/49905 en
dc.description.abstract Development of a vaccine to limit the impact of antibiotic resistant Neisseria gonorrhoeae is now a global priority. Serum bactericidal antibody (SBA) is a possible indicator of protective immunity to N. gonorrhoeae, but conventional assays measure colony forming units (CFU), which is time-consuming. A luminescent assay that quantifies ATP as a surrogate measure of bacterial viability was tested on N. gonorrhoeae strains FA1090, MS11 and P9-17 and compared to CFU-based readouts. There was a linear relationship between CFU and ATP levels for all three strains (r > 0.9). Normal human serum (NHS) is a common source of complement for SBA assays, but needs to be screened for non-specific bactericidal activity. NHS from 10 individuals were used for serum sensitivity assays—sensitivity values were significantly reduced with the ATP method for FA1090 (5/10, p < 0.05) and MS11 (10/10, p < 0.05), whereas P9-17 data were comparable for all donors. Our results suggest that measuring ATP underestimates serum sensitivity of N. gonorrhoeae and that the CFU method is a better approach. However, mouse anti-P9-17 outer membrane vesicles (OMV) SBA titres to P9-17 were comparable with both methods (r = 0.97), suggesting this assay can be used to rapidly screen sera for bactericidal antibodies to gonococci. en
dc.publisher MDPI AG en
dc.relation.ispartofseries Vaccines en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.title Feasibility of using a luminescence-based method to determine serum bactericidal activity against Neisseria gonorrhoeae en
dc.type Journal Article en
dc.identifier.doi 10.3390/vaccines7040191 en
pubs.issue 4 en
pubs.volume 7 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Article en
pubs.elements-id 788213 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Molecular Medicine en
pubs.org-id Pharmacology en
dc.identifier.eissn 2076-393X en
pubs.record-created-at-source-date 2020-01-14 en
pubs.dimensions-id 31766474 en

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