dc.contributor.author |
Hunter, Mandana |
en |
dc.contributor.author |
Demarais, Nicholas |
en |
dc.contributor.author |
Faull, Richard |
en |
dc.contributor.author |
Grey, Angus |
en |
dc.contributor.author |
Curtis, Maurice |
en |
dc.date.accessioned |
2020-02-13T03:00:26Z |
en |
dc.date.issued |
2018-09 |
en |
dc.identifier.citation |
Journal of neurochemistry 146(5):613-630 Sep 2018 |
en |
dc.identifier.issn |
0022-3042 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/49967 |
en |
dc.description.abstract |
The human subventricular zone (SVZ) has a defined cytological and neurochemical architecture, with four constituent laminae that act in concert to support its neurogenic activity. Lipidomic specialisation has previously been demonstrated in the neurologically normal human SVZ, with enrichment of functionally important lipid classes in each lamina. The SVZ is also responsive to neurodegenerative disorders, where thickening of the niche and enhanced proliferation of resident cells were observed in Huntington's disease (HD) brains. In this study, we hypothesised lipidomic changes in the HD SVZ. Using matrix-assisted laser desorption/ionisation (MALDI) imaging mass spectrometry, this analysis shows differences in the lipidomic architecture in the post-mortem Vonsattel grade III cases. Relative to matched, neurologically normal specimens (N = 4), the lipidomic signature of the HD SVZ (N = 4) was characterized by loss of sulfatides and triglycerides in the myelin layer, with an ectopic and focal accumulation of sphingomyelins and ceramide-1-phosphate observed in this lamina. A striking loss of lipidomic patterning was also observed in the ependymal layer, where the local abundance of phosphatidylinositols was significantly reduced in HD. This comprehensive spatially resolved lipidomic analysis of the human HD SVZ identifies alterations in lipid architecture that may shed light on the mechanisms of SVZ responses to neurodegeneration in HD. Open Science: This manuscript was awarded with the Open Materials Badge. For more information see: https://cos.io/our-services/open-science-badges/. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Journal of neurochemistry |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights |
This is the peer reviewed version of the following article: Journal of neurochemistry 146(5):613-630 Sep 2018, which has been published in final form at http://dx.doi.org/10.1111/jnc.14468. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://authorservices.wiley.com/author-resources/Journal-Authors/licensing-and-open-access/open-access/self-archiving.html |
en |
dc.subject |
Lateral Ventricles |
en |
dc.subject |
Humans |
en |
dc.subject |
Huntington Disease |
en |
dc.subject |
Phosphoric Monoester Hydrolases |
en |
dc.subject |
Sulfoglycosphingolipids |
en |
dc.subject |
Sphingomyelins |
en |
dc.subject |
Lipids |
en |
dc.subject |
Triglycerides |
en |
dc.subject |
Autopsy |
en |
dc.subject |
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
en |
dc.subject |
Fourier Analysis |
en |
dc.subject |
Adult |
en |
dc.subject |
Aged |
en |
dc.subject |
Middle Aged |
en |
dc.subject |
Female |
en |
dc.subject |
Male |
en |
dc.subject |
Lipid Metabolism |
en |
dc.subject |
Mass Spectrometry |
en |
dc.title |
Subventricular zone lipidomic architecture loss in Huntington's disease. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1111/jnc.14468 |
en |
pubs.issue |
5 |
en |
pubs.begin-page |
613 |
en |
pubs.volume |
146 |
en |
dc.rights.holder |
Copyright: International Society for Neurochemistry |
en |
pubs.end-page |
630 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
751443 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Anatomy and Medical Imaging |
en |
pubs.org-id |
Physiology Division |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Biological Sciences |
en |
dc.identifier.eissn |
1471-4159 |
en |
pubs.record-created-at-source-date |
2018-05-28 |
en |
pubs.dimensions-id |
29804301 |
en |