Subventricular zone lipidomic architecture loss in Huntington's disease.

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Show simple item record Hunter, Mandana en Demarais, Nicholas en Faull, Richard en Grey, Angus en Curtis, Maurice en 2020-02-13T03:00:26Z en 2018-09 en
dc.identifier.citation Journal of neurochemistry 146(5):613-630 Sep 2018 en
dc.identifier.issn 0022-3042 en
dc.identifier.uri en
dc.description.abstract The human subventricular zone (SVZ) has a defined cytological and neurochemical architecture, with four constituent laminae that act in concert to support its neurogenic activity. Lipidomic specialisation has previously been demonstrated in the neurologically normal human SVZ, with enrichment of functionally important lipid classes in each lamina. The SVZ is also responsive to neurodegenerative disorders, where thickening of the niche and enhanced proliferation of resident cells were observed in Huntington's disease (HD) brains. In this study, we hypothesised lipidomic changes in the HD SVZ. Using matrix-assisted laser desorption/ionisation (MALDI) imaging mass spectrometry, this analysis shows differences in the lipidomic architecture in the post-mortem Vonsattel grade III cases. Relative to matched, neurologically normal specimens (N = 4), the lipidomic signature of the HD SVZ (N = 4) was characterized by loss of sulfatides and triglycerides in the myelin layer, with an ectopic and focal accumulation of sphingomyelins and ceramide-1-phosphate observed in this lamina. A striking loss of lipidomic patterning was also observed in the ependymal layer, where the local abundance of phosphatidylinositols was significantly reduced in HD. This comprehensive spatially resolved lipidomic analysis of the human HD SVZ identifies alterations in lipid architecture that may shed light on the mechanisms of SVZ responses to neurodegeneration in HD. Open Science: This manuscript was awarded with the Open Materials Badge. For more information see: en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Journal of neurochemistry en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights This is the peer reviewed version of the following article: Journal of neurochemistry 146(5):613-630 Sep 2018, which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. en
dc.rights.uri en
dc.rights.uri en
dc.subject Lateral Ventricles en
dc.subject Humans en
dc.subject Huntington Disease en
dc.subject Phosphoric Monoester Hydrolases en
dc.subject Sulfoglycosphingolipids en
dc.subject Sphingomyelins en
dc.subject Lipids en
dc.subject Triglycerides en
dc.subject Autopsy en
dc.subject Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization en
dc.subject Fourier Analysis en
dc.subject Adult en
dc.subject Aged en
dc.subject Middle Aged en
dc.subject Female en
dc.subject Male en
dc.subject Lipid Metabolism en
dc.subject Mass Spectrometry en
dc.title Subventricular zone lipidomic architecture loss in Huntington's disease. en
dc.type Journal Article en
dc.identifier.doi 10.1111/jnc.14468 en
pubs.issue 5 en
pubs.begin-page 613 en
pubs.volume 146 en
dc.rights.holder Copyright: International Society for Neurochemistry en
pubs.end-page 630 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype Journal Article en
pubs.elements-id 751443 en Medical and Health Sciences en Medical Sciences en Anatomy and Medical Imaging en Physiology Division en Science en Biological Sciences en
dc.identifier.eissn 1471-4159 en
pubs.record-created-at-source-date 2018-05-28 en
pubs.dimensions-id 29804301 en

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