Detection of small molecule concentration gradients in ocular tissues and humours.

ResearchSpace/Manakin Repository

Show simple item record Boughton, Berin A en Thomas, Oliver RB en Demarais, Nicholas en Trede, Dennis en Swearer, Stephen E en Grey, Angus en 2020-02-13T03:40:52Z en 2019-10-26 en
dc.identifier.issn 1076-5174 en
dc.identifier.uri en
dc.description.abstract The eye is an elegant organ consisting of a number of tissues and fluids with specialised functions that together allow it to effectively transmit and transduce light input to the brain for visual perception. One key determinant of this integrated function is the spatial relationship of ocular tissues. Biomolecular distributions within the main ocular tissues cornea, lens, and retina have been studied extensively in isolation, yet the potential for metabolic communication between ocular tissues via the ocular humours has been difficult to visualise. To address this limitation, the current study presents a method to map spatial distributions of metabolites and small molecules in whole eyes, including ocular humours. Using a tape-transfer system and freeze-drying, the spatial distribution of ocular small molecules was investigated in mouse, rat, fish (black bream), and rabbit eyes using negative ion mode MALDI imaging mass spectrometry. Full-scan imaging was used for discovery experiments, while MS/MS imaging for identification and localisation was also demonstrated. In all eyes, metabolites such as glutathione and phospholipids were localised in the main ocular tissues. In addition, in rodent eyes, major metabolites were distributed relatively uniformly in ocular humours. In contrast, both uniform and spatially defined ocular metabolite distributions were observed in the black bream eye. Tissue and ocular humour distributions were reproducible, as demonstrated by the three-dimensional analysis of a mouse eye, and able to be captured with high spatial resolution analysis. The presented method could be used to further investigate the role of inter-tissue metabolism in ocular health, and to support the development of therapeutics to treat major ocular diseases. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Journal of mass spectrometry : JMS en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.title Detection of small molecule concentration gradients in ocular tissues and humours. en
dc.type Journal Article en
dc.identifier.doi 10.1002/jms.4460 en
dc.rights.holder Copyright: The author en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Journal Article en
pubs.elements-id 784814 en Medical and Health Sciences en Medical Sciences en Physiology Division en Science en Biological Sciences en
dc.identifier.eissn 1096-9888 en
pubs.record-created-at-source-date 2019-10-27 en
pubs.dimensions-id 31654531 en

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