Paired analysis of the microbiota between surface tissue swabs and biopsies from pediatric patients undergoing adenotonsillectomy.

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dc.contributor.author Johnston, James en
dc.contributor.author Hoggard, Michael en
dc.contributor.author Biswas, Kristi en
dc.contributor.author Astudillo Garcia, Maria del Carmen en
dc.contributor.author Radcliff, Fiona en
dc.contributor.author Mahadevan, Murali en
dc.contributor.author Douglas, Richard en
dc.date.accessioned 2020-02-13T03:41:25Z en
dc.date.issued 2018-10 en
dc.identifier.issn 0165-5876 en
dc.identifier.uri http://hdl.handle.net/2292/50004 en
dc.description.abstract INTRODUCTION:Culture-independent methods, based on bacterial 16 S rRNA gene sequencing, have been used previously to investigate the adenotonsillar microbiota. However, these studies have focused on a single sampling site (usually a surface swab). We aimed to investigate potential differences in adenotonsillar microbiota according to sampling location, both on and within the adenoids and palatine tonsils. METHODS:Pediatric patients (n = 28, mean age five years) undergoing adenotonsillectomy were recruited for this study. At the time of surgery, a mucosal adenoid surface swab and an adenoid tissue biopsy was collected. Immediately following surgery, the crypts of the right and left tonsils were swabbed, and a surface and core tissue sample from the right tonsil were also collected. Bacterial 16 S rRNA gene-targeted amplicon sequencing was used to determine the bacterial composition of the collected samples. RESULTS:There was no significant difference in diversity or composition of the adenoid microbiota based on sampling site. However, the Shannon-Wiener and Inverse-Simpson diversity indices differed significantly (p < 0.05) between the microbial communities of the three different tonsil sampling sites. There was a higher average relative abundance of members from the genera Streptococcus, Actinobacillus, and Neisseria in the tonsil crypts when compared with surface and core tonsil tissue samples. CONCLUSION:Our results indicate that there is variation in bacterial diversity and composition based on sampling sites in the tonsils but not the adenoids. The difference in microbiota between the surface and the tissue may have implications for our understanding of the pathogenesis of recurrent tonsillitis and have treatment implications. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries International journal of pediatric otorhinolaryngology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Adenoids en
dc.subject Humans en
dc.subject Tonsillitis en
dc.subject Biopsy en
dc.subject Adenoidectomy en
dc.subject Tonsillectomy en
dc.subject Adolescent en
dc.subject Child en
dc.subject Child, Preschool en
dc.subject Female en
dc.subject Male en
dc.subject Palatine Tonsil en
dc.subject Microbiota en
dc.title Paired analysis of the microbiota between surface tissue swabs and biopsies from pediatric patients undergoing adenotonsillectomy. en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.ijporl.2018.07.024 en
pubs.begin-page 51 en
pubs.volume 113 en
dc.rights.holder Copyright: The author en
pubs.end-page 57 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Journal Article en
pubs.elements-id 750404 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Molecular Medicine en
pubs.org-id School of Medicine en
pubs.org-id Surgery Department en
pubs.org-id Science en
pubs.org-id Biological Sciences en
dc.identifier.eissn 1872-8464 en
pubs.record-created-at-source-date 2018-09-04 en
pubs.dimensions-id 30174010 en


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