dc.contributor.author |
Johnston, James |
en |
dc.contributor.author |
Hoggard, Michael |
en |
dc.contributor.author |
Biswas, Kristi |
en |
dc.contributor.author |
Astudillo Garcia, Maria del Carmen |
en |
dc.contributor.author |
Radcliff, Fiona |
en |
dc.contributor.author |
Mahadevan, Murali |
en |
dc.contributor.author |
Douglas, Richard |
en |
dc.date.accessioned |
2020-02-13T03:41:25Z |
en |
dc.date.issued |
2018-10 |
en |
dc.identifier.issn |
0165-5876 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/50004 |
en |
dc.description.abstract |
INTRODUCTION:Culture-independent methods, based on bacterial 16 S rRNA gene sequencing, have been used previously to investigate the adenotonsillar microbiota. However, these studies have focused on a single sampling site (usually a surface swab). We aimed to investigate potential differences in adenotonsillar microbiota according to sampling location, both on and within the adenoids and palatine tonsils. METHODS:Pediatric patients (n = 28, mean age five years) undergoing adenotonsillectomy were recruited for this study. At the time of surgery, a mucosal adenoid surface swab and an adenoid tissue biopsy was collected. Immediately following surgery, the crypts of the right and left tonsils were swabbed, and a surface and core tissue sample from the right tonsil were also collected. Bacterial 16 S rRNA gene-targeted amplicon sequencing was used to determine the bacterial composition of the collected samples. RESULTS:There was no significant difference in diversity or composition of the adenoid microbiota based on sampling site. However, the Shannon-Wiener and Inverse-Simpson diversity indices differed significantly (p < 0.05) between the microbial communities of the three different tonsil sampling sites. There was a higher average relative abundance of members from the genera Streptococcus, Actinobacillus, and Neisseria in the tonsil crypts when compared with surface and core tonsil tissue samples. CONCLUSION:Our results indicate that there is variation in bacterial diversity and composition based on sampling sites in the tonsils but not the adenoids. The difference in microbiota between the surface and the tissue may have implications for our understanding of the pathogenesis of recurrent tonsillitis and have treatment implications. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
International journal of pediatric otorhinolaryngology |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Adenoids |
en |
dc.subject |
Humans |
en |
dc.subject |
Tonsillitis |
en |
dc.subject |
Biopsy |
en |
dc.subject |
Adenoidectomy |
en |
dc.subject |
Tonsillectomy |
en |
dc.subject |
Adolescent |
en |
dc.subject |
Child |
en |
dc.subject |
Child, Preschool |
en |
dc.subject |
Female |
en |
dc.subject |
Male |
en |
dc.subject |
Palatine Tonsil |
en |
dc.subject |
Microbiota |
en |
dc.title |
Paired analysis of the microbiota between surface tissue swabs and biopsies from pediatric patients undergoing adenotonsillectomy. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.ijporl.2018.07.024 |
en |
pubs.begin-page |
51 |
en |
pubs.volume |
113 |
en |
dc.rights.holder |
Copyright: The author |
en |
pubs.end-page |
57 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
750404 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Surgery Department |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Biological Sciences |
en |
dc.identifier.eissn |
1872-8464 |
en |
pubs.record-created-at-source-date |
2018-09-04 |
en |
pubs.dimensions-id |
30174010 |
en |