Resistance to neomycin ototoxicity in the extreme basal (hook) region of the mouse cochlea.

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dc.contributor.author Lin, Ching Yu en
dc.contributor.author Thorne, Peter en
dc.contributor.author Housley, Gary D en
dc.contributor.author Vlajkovic, Srdjan en
dc.date.accessioned 2020-02-17T01:25:29Z en
dc.date.issued 2018-09 en
dc.identifier.issn 0948-6143 en
dc.identifier.uri http://hdl.handle.net/2292/50067 en
dc.description.abstract Aminoglycoside ototoxicity results in permanent loss of the sensory hair cells in the mammalian cochlea. It usually begins at the basal turn causing high-frequency hearing loss. Here we describe previously unreported resistance of hair cells to neomycin ototoxicity in the extreme basal (hook) region of the developing cochlea of the C57BL/6 mouse. Organ of Corti explants from mice at postnatal day 3 were incubated (37 °C, 5% CO2) in normal culture medium for 19.5 h prior to and after exposure to neomycin (1 mM, 3 h). To study neomycin uptake in the hair cells, cochlear explants were incubated with Neomycin Texas-red (NTR) conjugate. As expected, exposure to neomycin significantly reduced the survival of inner (IHC) and outer hair cells (OHC). IHC survival rate was high in the apical segment and low in the basal segment. OHC were well preserved in the apical and hook regions, with substantial OHC loss in the basal segment. The NTR uptake study demonstrated that the high survival rate in the extreme basal turn OHC was associated with low NTR uptake. Treatment with a calcium chelator (BAPTA), which disrupts the opening of mechanoelectrical (MET) transduction channels, abolished or reduced NTR uptake in the hair cells throughout the cochlea. This confirmed the essential role of MET channels in neomycin uptake and implied that the transduction channels could be impaired in the hook region of the developing mouse cochlea, possibly as a result of the cadherin 23 mutation responsible for the progressive deafness in C57BL/6 mice. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Histochemistry and cell biology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Cochlea en
dc.subject Animals en
dc.subject Mice, Inbred C57BL en
dc.subject Mice en
dc.subject Neomycin en
dc.subject Organ Culture Techniques en
dc.subject Tissue Distribution en
dc.subject Hair Cells, Auditory, Outer en
dc.title Resistance to neomycin ototoxicity in the extreme basal (hook) region of the mouse cochlea. en
dc.type Journal Article en
dc.identifier.doi 10.1007/s00418-018-1683-8 en
pubs.issue 3 en
pubs.begin-page 281 en
pubs.volume 150 en
dc.rights.holder Copyright: The author en
pubs.end-page 289 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Journal Article en
pubs.elements-id 744805 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Physiology Division en
pubs.org-id Population Health en
pubs.org-id Audiology en
pubs.org-id Science en
pubs.org-id Biological Sciences en
dc.identifier.eissn 1432-119X en
pubs.record-created-at-source-date 2018-06-05 en
pubs.dimensions-id 29862415 en


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