dc.contributor.author |
Hughes, Jacelyn |
en |
dc.contributor.author |
Proft, Thomas |
en |
dc.date.accessioned |
2016-02-09T03:37:33Z |
en |
dc.date.accessioned |
2020-04-03T02:08:50Z |
en |
dc.date.available |
2015-09-22 |
en |
dc.date.issued |
2016-01 |
en |
dc.identifier.citation |
Biotechnology letters, 2016, 38 (1), pp. 139 - 143 |
en |
dc.identifier.issn |
0141-5492 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/50233 |
en |
dc.description.abstract |
Objective: To determine if multiple streptococcal species can be easily labeled for biophotonic imaging using a toxin-antitoxin stabilized reporter plasmid containing the native firefly luciferase gene, originally developed for use in Group A Streptococcus. Results: A number of streptococcal species including Group B Streptococcus, Group C Streptococcus, Group G Streptococcus, S. iniae, S. vestibularis, and S. salivarius were successfully transformed with the reporter plasmid. In absence of antibiotic selection, the plasmid had variable stability amongst the six strains. The expression of firefly luciferase was highest in Group B Streptococcus and S. iniae, as observed by the brightest signal and lowest detection limits in vitro. Conclusion: Multiple streptococcal species can be easily transformed with our toxin-antitoxin stabilized bioluminescent reporter plasmid. However, this plasmid shows variable stability and signal in different species, restricting its use for certain applications. |
en |
dc.format.medium |
Print-Electronic |
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dc.language |
English |
en |
dc.relation.ispartofseries |
Biotechnology letters |
en |
dc.relation.replaces |
http://hdl.handle.net/2292/28220 |
en |
dc.relation.replaces |
2292/28220 |
en |
dc.relation.replaces |
2292/38490 |
en |
dc.relation.replaces |
http://hdl.handle.net/2292/38490 |
en |
dc.relation.replaces |
2292/38490 |
en |
dc.relation.replaces |
http://hdl.handle.net/2292/38490 |
en |
dc.relation.replaces |
2292/38490 |
en |
dc.relation.replaces |
http://hdl.handle.net/2292/38490 |
en |
dc.relation.replaces |
2292/38490 |
en |
dc.relation.replaces |
http://hdl.handle.net/2292/38490 |
en |
dc.relation.replaces |
2292/38490 |
en |
dc.relation.replaces |
http://hdl.handle.net/2292/38490 |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0141-5492/
http://www.springer.com/gp/open-access/authors-rights/self-archiving-policy/2124 |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Bioluminescence |
en |
dc.subject |
Biophotonic imaging |
en |
dc.subject |
Firefly luciferase |
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dc.subject |
Streptococcus spp. |
en |
dc.subject |
Toxin-antitoxin system |
en |
dc.title |
Stabilized plasmid-based system for bioluminescent labeling of multiple streptococcal species |
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dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1007/s10529-015-1967-1 |
en |
pubs.issue |
1 |
en |
pubs.begin-page |
139 |
en |
pubs.volume |
38 |
en |
dc.identifier.pmid |
26410784 |
en |
pubs.author-url |
http://link.springer.com/article/10.1007/s10529-015-1967-1 |
en |
pubs.end-page |
143 |
en |
pubs.merge-from |
2292/38490 |
en |
pubs.merge-from |
http://hdl.handle.net/2292/38490 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
501517 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1573-6776 |
en |
pubs.record-created-at-source-date |
2016-02-09 |
en |
pubs.dimensions-id |
26410784 |
en |