dc.contributor.author |
Khalifa, ME |
en |
dc.contributor.author |
MacDiarmid, Robin |
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dc.date.accessioned |
2020-04-09T00:44:54Z |
en |
dc.date.issued |
2019-10-11 |
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dc.identifier.citation |
Frontiers in Microbiology 10:9 pages Article number 2318 11 Oct 2019 |
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dc.identifier.issn |
1664-302X |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/50311 |
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dc.description.abstract |
Mycoviruses are widely distributed across different phyla of the fungal kingdom. Viruses that share significant sequence similarities have been reported in different fungi, suggesting descent from a common ancestor. In this study, two fungal genera isolated from the same sample, Trichoderma koningiopsis isolate Mg10 and Clonostachys rosea isolate Mg06, were reported to have identical double-stranded RNA (dsRNA) profiles that consist of two virus-like, dsRNA elements (dsRNA-L and dsRNA-S). The complete sequence and genome organization of dsRNA-L from isolate Mg10 was determined. It is 4712 nucleotides (nt) long and contains two non-overlapping open reading frames (ORFs) that code for proteins with similarities to totiviruses. Consequently the virus was given the proposed name Trichoderma koningiopsis totivirus 1 (TkTV1/Mg10). The TkTV1/Mg10 genome structure resembles that of yeast totiviruses in which the region preceding the stop codon of ORF1 contains the elements required for -1 ribosomal frameshifting which may induce the expression of an ORF1–ORF2 (CP-RdRp) fusion protein. Sequence analyses of viral dsRNA-L from C. rosea isolate Mg06 revealed that it is nearly identical with that of TkTV1/Mg10. This relatedness was confirmed by northern blot hybridization and indicates very recent natural horizontal transmission of this virus between unrelated fungi. TkTV1 purified isometric virions were ∼38–40 nm in diameter and were able to transfect T. koningiopsis and C. rosea protoplasts. This is another report of a mycovirus present naturally in two taxonomically distinct fungi. |
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dc.publisher |
Frontiers Media |
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dc.relation.ispartofseries |
Frontiers in Microbiology |
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dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
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dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
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dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
en |
dc.rights.uri |
https://www.frontiersin.org/about/open-access |
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dc.title |
A Novel Totivirus Naturally Occurring in Two Different Fungal Genera |
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dc.type |
Journal Article |
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dc.identifier.doi |
10.3389/fmicb.2019.02318 |
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pubs.volume |
10 |
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dc.rights.holder |
Copyright: The author |
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pubs.author-url |
https://www.frontiersin.org/articles/10.3389/fmicb.2019.02318/full |
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pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
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pubs.subtype |
Article |
en |
pubs.elements-id |
785192 |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Biological Sciences |
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dc.identifier.eissn |
1664-302X |
en |
pubs.number |
2318 |
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pubs.record-created-at-source-date |
2020-05-28 |
en |