Identifying alleles of carotenoid pathway genes controlling yellow flesh phenotype in apples

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dc.contributor.advisor Allan, A en
dc.contributor.advisor Ampomah-Dwamena, C en
dc.contributor.advisor Espley, R en
dc.contributor.author Bhargava, Nitisha en
dc.date.accessioned 2020-06-30T02:07:48Z en
dc.date.issued 2020 en
dc.identifier.uri http://hdl.handle.net/2292/51752 en
dc.description Full Text is available to authenticated members of The University of Auckland only. en
dc.description.abstract Carotenoids are lipophilic isoprenoid compounds responsible for yellow and orange pigmentation in fruits and vegetables. In plants, they protect the photosynthetic unit from photo-oxidative stress and are precursors to two phytohormones, abscisic acid, and strigolactones. Consumption of these secondary metabolites has been associated with reducing the risk of diseases like prostate cancer and age-related macular degeneration. Apple is one of the most significant fruit crops globally. The skin of the fruit accumulates high levels of anthocyanins and carotenoids. However, the flesh has low levels of carotenoids compared to the skin. Increasing carotenoids in the flesh of apple will increase its nutritional value as flesh occupies most of the fruit space. A cross between ‘Aotea’ cultivar (small fruit with high carotenoids and yellow flesh) and ‘Malling 9’ (large fruit with low carotenoids and white flesh) yielded progenies with varying flesh colour and carotenoid levels. The population was utilized to understand the genetic control of carotenoid biosynthesis in apple flesh. Single nucleotide polymorphism (SNP) genotyping was conducted to identify the segregating alleles of carotenoid biosynthesis pathway genes from ‘Aotea’ in the population. Candidate genes were selected based on the allelic analysis, regression analysis, and published literature. Expression analysis of the candidate gene isopentenyl pyrophosphate (IPPI) in yellow and white-fleshed cultivars revealed high expression at an early stage of fruit development, which may influence carotenoid concentration in the flesh. Functional analysis revealed the potential role of phytoene synthase 4 (PSY4) in regulating the first committed step of the carotenoid biosynthesis pathway in yellow-fleshed cultivars. These findings provide insights into understanding the carotenoid biosynthesis pathway in the flesh of yellow apple cultivars in order to increase the carotenoid concentration in commercial cultivars, and thereby increasing fruit nutritional value. en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof Masters Thesis - University of Auckland en
dc.relation.isreferencedby UoA en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights Restricted Item. Full Text is available to authenticated members of The University of Auckland only. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/nz/ en
dc.title Identifying alleles of carotenoid pathway genes controlling yellow flesh phenotype in apples en
dc.type Thesis en
thesis.degree.discipline Biological Sciences en
thesis.degree.grantor The University of Auckland en
thesis.degree.level Masters en
dc.rights.holder Copyright: The author en
pubs.elements-id 804779 en
pubs.record-created-at-source-date 2020-06-30 en


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