A novel chrysovirus from a clinical isolate of Aspergillus thermomutatus affects sporulation.

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dc.contributor.author Ejmal, Mahjoub A en
dc.contributor.author Holland, David J en
dc.contributor.author MacDiarmid, Robin en
dc.contributor.author Pearson, Michael en
dc.date.accessioned 2020-07-07T00:56:44Z en
dc.date.issued 2018-01 en
dc.identifier.citation PloS one 13(12):e0209443 Jan 2018 en
dc.identifier.issn 1932-6203 en
dc.identifier.uri http://hdl.handle.net/2292/51909 en
dc.description.abstract A clinical isolate of Aspergillus thermomutatus (Teleomorph: Neosartorya pseudofischeri) was found to contain ~35 nm isometric virus-like particles associated with four double-stranded (ds) RNA segments, each of which coded for a single open reading frame. The longest dsRNA element (3589 nt) encodes a putative RNA-dependent RNA polymerase (1114 aa), the second longest dsRNA element (2772 nt) encodes a coat protein (825 aa), and the other two dsRNAs (2676 nt, 2514 nt) encode hypothetical proteins of 768 aa and 711 aa, respectively. Phylogenetic analysis of the amino acid sequences showed 41-60% similarity to the proteins coded by the dsRNAs of the most closely related virus, Penicillium janczewskii chrysovirus 2, indicating that it is a new species based on the International Committee on Taxonomy of Viruses criteria for the genus Chrysovirus. This is the first virus reported from A. thermomutatus and was tentatively named Aspergillus thermomutatus chrysovirus 1. A virus free line of the fungal isolate, cured by cycloheximide treatment, produced large numbers of conidia but no ascospores at both 20°C and 37°C, whereas the virus infected line produced ten-fold fewer conidia at 20°C and a large number of ascospores at both temperatures. The effects of the virus on fungal sporulation have interesting implications for the spread of the fungus and possible use of the virus as a biological control agent. en
dc.format.medium Electronic-eCollection en
dc.language eng en
dc.relation.ispartofseries PloS one en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Humans en
dc.subject Aspergillus en
dc.subject Aspergillosis en
dc.subject RNA, Double-Stranded en
dc.subject RNA, Viral en
dc.subject Phylogeny en
dc.subject Biological Control Agents en
dc.subject Fungal Viruses en
dc.title A novel chrysovirus from a clinical isolate of Aspergillus thermomutatus affects sporulation. en
dc.type Journal Article en
dc.identifier.doi 10.1371/journal.pone.0209443 en
pubs.issue 12 en
pubs.begin-page e0209443 en
pubs.volume 13 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.elements-id 758859 en
pubs.org-id Science en
pubs.org-id Biological Sciences en
dc.identifier.eissn 1932-6203 en
pubs.record-created-at-source-date 2018-12-21 en
pubs.dimensions-id 30571748 en

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