dc.contributor.author |
Khemlani, AHJ |
en |
dc.contributor.author |
Proft, Thomas |
en |
dc.contributor.author |
Loh, JMS |
en |
dc.contributor.editor |
Proft, T |
en |
dc.contributor.editor |
Loh, J |
en |
dc.date.accessioned |
2020-07-07T02:29:45Z |
en |
dc.date.issued |
2020-01 |
en |
dc.identifier.isbn |
978-1-0716-0467-0 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/51935 |
en |
dc.description.abstract |
The critical first step of Group A Streptococcus (GAS) pathogenesis is adhesion to the host pharyngeal and skin epithelial cell surfaces (Brouwer et al., FEBS Lett 590:3739-3757, 2016). Host-cell adhesion assays provide a straightforward model to study these host-pathogen interactions. Here, we describe the culturing of immortalized cell lines into monolayers to mimic host epithelia. Various GAS strains can then be added to study their adhesion properties. In addition, we describe the use of antibodies raised against the cell-surface components of GAS to study if these are able to neutralize the binding of GAS to the cell lines. This provides an indication if these cell-surface components are involved in adhesion and if antibodies generated against them function through neutralization. |
en |
dc.format.medium |
Print |
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dc.publisher |
Springer |
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dc.relation.ispartof |
Group A Streptococcus |
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dc.relation.ispartofseries |
Methods in Molecular Biology |
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dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
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dc.title |
Assays to Analyze Adhesion of Group A Streptococcus to Host Cells. |
en |
dc.type |
Book Item |
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dc.identifier.doi |
10.1007/978-1-0716-0467-0_20 |
en |
pubs.begin-page |
271 |
en |
pubs.volume |
2136 |
en |
dc.rights.holder |
Copyright: The author |
en |
pubs.end-page |
278 |
en |
pubs.place-of-publication |
New York, USA |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.elements-id |
803162 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
pubs.number |
20 |
en |
pubs.record-created-at-source-date |
2020-07-09 |
en |
pubs.dimensions-id |
32430828 |
en |