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| dc.contributor.author | Loh, JMS | en |
| dc.contributor.author | Soh, KY | en |
| dc.contributor.author | Proft, Thomas | en |
| dc.contributor.editor | Proft, T | en |
| dc.contributor.editor | Loh, J | en |
| dc.date.accessioned | 2020-07-07T02:29:50Z | en |
| dc.date.issued | 2020-01 | en |
| dc.identifier.isbn | 978-1-0716-0467-0 | en |
| dc.identifier.uri | http://hdl.handle.net/2292/51936 | en |
| dc.description.abstract | Bioluminescence is a rapid and cost-saving technology that enables monitoring of bacteria in real time in animal infection models. Here, we report a method for labeling GAS with a set of plasmids we have developed and deposited with Addgene. These plasmids can be used to either integrate firefly luciferase (Ffluc) or red-shifted firefly luciferase (FflucRT) into the GAS genome or to introduce the reporters on plasmids which have been stabilized with a toxin-antitoxin system to prevent loss of plasmid in the absence of antibiotic selection. | en |
| dc.format.medium | en | |
| dc.publisher | Springer | en |
| dc.relation.ispartof | Group A Streptococcus | en |
| dc.relation.ispartofseries | Methods in Molecular Biology | en |
| dc.rights | Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. | en |
| dc.rights.uri | https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm | en |
| dc.title | Generation of Bioluminescent Group A Streptococcus for Biophotonic Imaging. | en |
| dc.type | Book Item | en |
| dc.identifier.doi | 10.1007/978-1-0716-0467-0_6 | en |
| pubs.begin-page | 71 | en |
| pubs.volume | 2136 | en |
| dc.rights.holder | Copyright: The author | en |
| pubs.end-page | 77 | en |
| pubs.place-of-publication | New York, USA | en |
| pubs.publication-status | Published | en |
| dc.rights.accessrights | http://purl.org/eprint/accessRights/RestrictedAccess | en |
| pubs.elements-id | 803164 | en |
| pubs.org-id | Medical and Health Sciences | en |
| pubs.org-id | Medical Sciences | en |
| pubs.org-id | Molecular Medicine | en |
| pubs.org-id | Science | en |
| pubs.org-id | Science Research | en |
| pubs.org-id | Maurice Wilkins Centre (2010-2014) | en |
| pubs.number | 6 | en |
| pubs.record-created-at-source-date | 2020-07-09 | en |
| pubs.dimensions-id | 32430814 | en |
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