Lipopolysaccharide-induced changes in the neurovascular unit in the preterm fetal sheep brain

Show simple item record Disdier, C en Awa, F en Chen, X en Dhillon, SK en Galinsky, R en Davidson, Joanne en Lear, Christopher en Bennet, Laura en Gunn, Alistair en Stonestreet, BS en 2020-07-07T22:11:19Z en 2020-05-28 en
dc.identifier.citation Journal of Neuroinflammation 17(1):14 pages Article number 167 28 May 2020 en
dc.identifier.uri en
dc.description.abstract Background: Exposure to inflammation during pregnancy can predispose to brain injury in premature infants. In the present study, we investigated the effects of prolonged exposure to inflammation on the cerebrovasculature of preterm fetal sheep. Methods: Chronically instrumented fetal sheep at 103-104 days of gestation (full term is ~ 147 days) received continuous low-dose lipopolysaccharide (LPS) infusions (100 ng/kg over 24 h, followed by 250 ng/kg/24 h for 96 h plus boluses of 1 μg LPS at 48, 72, and 96 h) or the same volume of normal saline (0.9%, w/v). Ten days after the start of LPS exposure at 113-114 days of gestation, the sheep were killed, and the fetal brain perfused with formalin in situ. Vessel density, pericyte and astrocyte coverage of the blood vessels, and astrogliosis in the cerebral cortex and white matter were determined using immunohistochemistry. Results: LPS exposure reduced (P < 0.05) microvascular vessel density and pericyte vascular coverage in the cerebral cortex and white matter of preterm fetal sheep, and increased the activation of perivascular astrocytes, but decreased astrocytic vessel coverage in the white matter. Conclusions: Prolonged exposure to LPS in preterm fetal sheep resulted in decreased vessel density and neurovascular remodeling, suggesting that chronic inflammation adversely affects the neurovascular unit and, therefore, could contribute to long-term impairment of brain development. en
dc.publisher BioMed Central en
dc.relation.ispartofseries Journal of Neuroinflammation en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
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dc.rights.uri en
dc.title Lipopolysaccharide-induced changes in the neurovascular unit in the preterm fetal sheep brain en
dc.type Journal Article en
dc.identifier.doi 10.1186/s12974-020-01852-y en
pubs.issue 1 en
pubs.volume 17 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 803747 en Medical and Health Sciences en Medical Sciences en Physiology Division en
dc.identifier.eissn 1742-2094 en
pubs.number 167 en
pubs.record-created-at-source-date 2020-06-11 en
pubs.dimensions-id 32466771 en

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