The effect of age on the microarchitecture and profile of gene expression in femoral head and neck bone from patients with osteoarthritis.

Show simple item record Naot, Dorit en Watson, Maureen en Choi, Ally J en Musson, David en Callon, Karen E en Zhu, Mark en Gao, Ryan en Caughey, William en Pitto, Rocco en Munro, Jacob en Horne, Anne en Gamble, Gregory en Dalbeth, Nicola en Reid, Ian en Cornish, Jillian en 2020-07-09T21:31:55Z en 2020-12 en
dc.identifier.citation Bone Reports 13 01 Dec 2020 en
dc.identifier.issn 2352-1872 en
dc.identifier.uri en
dc.description.abstract Ageing of the skeleton is characterised by decreased bone mineral density, reduced strength, and increased risk of fracture. Although it is known that these changes are determined by the activities of bone cells through the processes of bone modelling and remodelling, details of the molecular mechanisms that underlie age-related changes in bone are still missing. Here, we analysed age-related changes in bone microarchitecture along with global gene expression in samples obtained from patients with osteoarthritis (OA). We hypothesised that changes would be evident in both microarchitecture and gene expression and aimed to identify novel molecular mechanisms that underlie ageing processes in bone. Samples of femoral head and neck were obtained from patients undergoing hip arthroplasty for OA, who were either ≤60 years or ≥70 years of age. Bone microarchitecture was analysed in cores of trabecular bone from the femoral head (17 from the younger group and 18 from the older), and cortical bone from the femoral neck (25 younger/22 older), using a Skyscan 1172 microCT scanner (Bruker). Gene expression was compared between the two age groups in 20 trabecular samples from each group, and 10 cortical samples from each group, using Clariom S Human microarrays (ThermoFisher Scientific). We found no significant changes between the two age groups in indices of trabecular or cortical bone microarchitecture. Gene expression analysis identified seven genes that had higher expression in the older group, including the transcription factor EGR1 and the glucose transporter SLC2A3 (GLUT3), and 21 differentially expressed genes in cortical bone samples (P<0.05, fold change>2). However, none of the comparisons of gene expression had false discovery rate-adjusted P<0.1. In contrast to our working hypothesis, we found only minor differences in gene expression and no differences in bone microarchitecture between the two age-groups. It is possible that pathological processes related to OA provide protection against age-related changes in bone. Our study suggests that in patients with OA, the bone properties measured here in femoral head and neck do not deteriorate significantly from the sixth to the eighth decade of life. en
dc.format.medium Electronic-eCollection en
dc.language eng en
dc.relation.ispartofseries Bone reports en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.rights.uri en
dc.title The effect of age on the microarchitecture and profile of gene expression in femoral head and neck bone from patients with osteoarthritis. en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.bonr.2020.100287 en
pubs.begin-page 100287 en
pubs.volume 13 en
dc.rights.holder Copyright: The authors en
pubs.publication-status Accepted en
dc.rights.accessrights en
pubs.subtype research-article en
pubs.subtype Journal Article en
pubs.elements-id 804187 en Bioengineering Institute en ABI Associates en Medical and Health Sciences en School of Medicine en Medicine Department en Ophthalmology Department en Surgery Department en Science en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 2352-1872 en
pubs.record-created-at-source-date 2020-06-20 en
pubs.dimensions-id 32551338 en

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