The shear stress response of the syncytiotrophoblast

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dc.contributor.advisor Clark, A en
dc.contributor.advisor James, J en Moulvi, Ali en 2020-07-20T03:35:41Z en 2020 en
dc.identifier.uri en
dc.description Full Text is available to authenticated members of The University of Auckland only. en
dc.description.abstract The placenta is a fetal organ that nourishes the unborn baby within its mother’s womb and supports it through the most critical developmental phase of its life. However, in spite of the cardinal role the placenta plays in ensuring fetal health, our understanding of normal, and abnormal, placental development is nebulous, with the determinants of placental development not yet fully defined. This thesis investigates whether shear stress exerted upon the placental surface; the syncytiotrophoblast, as a result of fluidic flow around the placenta is one such determinant that regulates placentogenesis. This thesis reports the in-vivo expression of mechanosensitive proteins; Dynein-2, IFT88, Kinesin-2, Piezo1, Polycystin-2 and TRPV6 in the syncytiotrophoblast of fresh frozen tissue derived from first trimester (n=3), normal term (n=3) and pathological FGR (n-3) placentae. A corresponding in-vitro expression of Dynein-2, IFT88, Kinesin-2, Polycystin-2 and TRPV6 is reported in a first trimester culture of syncytiotrophoblasts. Additionally, first trimester (n=5) and term (n=5) tissue is imaged in 3D via micro-computerized tomography, to enable quantitative blood flow simulations that can predict the shear stress exerted upon the syncytiotrophoblast across gestation. Together, this thesis provides novel evidences that the syncytiotrophoblast, across gestation, and in pathological cases, is capable of mechanosensation. Furthermore, it validates and enables future experiments that can directly assess the response of the syncytiotrophoblast to physiological shear stresses predicted by mathematical models. en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof Masters Thesis - University of Auckland en
dc.relation.isreferencedby UoA en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights Restricted Item. Full Text is available to authenticated members of The University of Auckland only. en
dc.rights.uri en
dc.rights.uri en
dc.title The shear stress response of the syncytiotrophoblast en
dc.type Thesis en Engineering en The University of Auckland en Masters en
dc.rights.holder Copyright: The author en
pubs.elements-id 805903 en Campus Life en Accommodation Halls en
pubs.record-created-at-source-date 2020-07-20 en

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