dc.contributor.author |
Santos Samuel A |
en |
dc.contributor.author |
Vidigal Pedro MP |
en |
dc.contributor.author |
Guimaraes Lucio MS |
en |
dc.contributor.author |
Mafia Reginaldo G |
en |
dc.contributor.author |
Templeton Matthew D |
en |
dc.contributor.author |
Alfenas Acelino C |
en |
dc.date.accessioned |
2020-10-16T03:28:49Z |
|
dc.date.available |
2020-10-16T03:28:49Z |
|
dc.date.issued |
2020-7-7 |
en |
dc.identifier.issn |
0167-4412 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/53350 |
|
dc.description.abstract |
© 2020, Springer Nature B.V. Abstract: Key MessageA resistantE. grandisgenotype showed a constitutive overexpression of genes related to resistance to myrtle rust caused byA. psidii. Abstract Myrtle rust caused by Austropuccinia psidii is considered one of the most important fungal diseases affecting Eucalyptus spp. plantations in Brazil. Although the selection and planting of resistant eucalypt genotypes have been the major strategies to manage the disease in Brazil, the molecular mechanisms involved in resistance are still unclear. In this study, we evaluated the gene expression profile of two contrasting Eucalyptus grandis genotypes in resistance level to rust by RNA-Seq. The two genotypes showed a very different background gene expression level even without A. psidii infection. The resistant genotype had a constitutive overexpression of a large number of protein-coding genes compared to the susceptible genotype. These genes were mainly associated with signal transduction, photosynthesis, regulation and response to salicylic acid (SA), and protein kinase leucine-rich receptors (PK-LRR). PK-LRR and SA mediated disease resistance are well known to be effective against obligate biotroph pathogens, such as A. psidii. In addition, at 24 h after infection, the susceptible genotype was able to activate some response, however, several resistance-related proteins had their expression level reduced with A. psidii infection. Here, we present the first analysis of E. grandis genotypes transcriptomes infected by A. psidii and it reveals a constitutive overexpression of several resistance-related genes in the resistant genotype compared to the susceptible one. Our findings have the potential to be used as candidate molecular markers for resistance to myrtle rust. |
en |
dc.language |
English |
en |
dc.publisher |
SPRINGER |
en |
dc.relation.ispartofseries |
PLANT MOLECULAR BIOLOGY |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Science & Technology |
en |
dc.subject |
Life Sciences & Biomedicine |
en |
dc.subject |
Biochemistry & Molecular Biology |
en |
dc.subject |
Plant Sciences |
en |
dc.subject |
Myrtle rust |
en |
dc.subject |
RNA-Seq |
en |
dc.subject |
DEGs |
en |
dc.subject |
Protein kinase |
en |
dc.subject |
Leucine-rich receptors |
en |
dc.subject |
LEUCINE-RICH REPEAT |
en |
dc.subject |
PUCCINIA-PSIDII |
en |
dc.subject |
DISEASE-RESISTANCE |
en |
dc.subject |
EUCALYPTUS-GRANDIS |
en |
dc.subject |
RNA-SEQ |
en |
dc.subject |
DEFENSE RESPONSES |
en |
dc.subject |
SALICYLIC-ACID |
en |
dc.subject |
MYRTLE RUST |
en |
dc.subject |
PROTEIN |
en |
dc.subject |
EXPRESSION |
en |
dc.subject |
0604 Genetics |
en |
dc.subject |
0607 Plant Biology |
en |
dc.subject |
0601 Biochemistry And Cell Biology |
en |
dc.title |
Transcriptome analysis ofEucalyptus grandisgenotypes reveals constitutive overexpression of genes related to rust (Austropuccinia psidii) resistance |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1007/s11103-020-01030-x |
en |
dc.date.updated |
2020-09-29T02:57:09Z |
en |
dc.rights.holder |
Copyright: The author |
en |
pubs.author-url |
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000546674700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e41486220adb198d0efde5a3b153e7d |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.subtype |
Early Access |
en |
pubs.subtype |
Journal |
en |
pubs.elements-id |
809436 |
en |
dc.identifier.eissn |
1573-5028 |
en |