Effect of the Extracellular Vesicle RNA Cargo From Uropathogenic Escherichia coli on Bladder Cells

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dc.contributor.author Dauros-Singorenko P
dc.contributor.author Hong J
dc.contributor.author Swift S
dc.contributor.author Phillips A
dc.contributor.author Blenkiron C
dc.date.accessioned 2020-11-18T03:30:58Z
dc.date.available 2020-11-18T03:30:58Z
dc.date.issued 2020-9-25
dc.identifier.citation Frontiers in molecular biosciences 7:580913 Jan 2020
dc.identifier.uri http://hdl.handle.net/2292/53641
dc.description.abstract Iron restriction in mammals, part of innate antimicrobial defense, may be sensed as a signal by an infecting pathogen. Iron-dependent regulators not only activate the pathogen’s specific iron acquisition and storage mechanisms needed for survival but also influence a number of other processes. Bacterial extracellular vesicles (EVs) are a conserved communication mechanism, which can have roles in host colonization, transfer of antimicrobial resistance, modulation of the host’s immune response, and biofilm formation. Here we analyze the iron-responsive effect of RNA cargo from Escherichia coli EVs in bladder cells. No differences were found in total RNA quantified from EVs released from representative pathogenic and probiotic strains grown in different iron conditions; nevertheless, lipopolysaccharide (LPS) associated with purified RNA was 10 times greater from EVs derived from the pathogenic strain. The pathogen and probiotic EV-RNA have no substantial toxic effect on the viability of cultured bladder cells, regardless of the iron concentration during bacterial culture. Transcriptomic analysis of bladder cells treated with pathogen EV-RNA delivered in artificial liposomes revealed a gene expression profile with a strong similarity to that of cells treated with liposomes containing LPS alone, with the majority being immune response pathways. EV-RNA from the probiotic strain gave no significant perturbation of gene expression in bladder cells. Cytokine profiling showed that EV-LPS has a role modulating the immune response when internalized by bladder cells, highlighting a key factor that must be considered when evaluating functional studies of bacterial RNA.
dc.relation.ispartofseries Frontiers in Molecular Biosciences
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.title Effect of the Extracellular Vesicle RNA Cargo From Uropathogenic Escherichia coli on Bladder Cells
dc.type Journal Article
dc.identifier.doi 10.3389/fmolb.2020.580913
pubs.volume 7
dc.date.updated 2020-10-20T20:49:45Z
dc.rights.holder Copyright: The authors en
pubs.author-url https://www.frontiersin.org/articles/10.3389/fmolb.2020.580913/full
pubs.publication-status Published
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Journal Article
pubs.elements-id 819901
dc.identifier.eissn 2296-889X

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https://creativecommons.org/licenses/by/4.0/ Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/


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