dc.contributor.author |
Jayaraman Jay |
|
dc.contributor.author |
Jones William T |
|
dc.contributor.author |
Harvey Dawn |
|
dc.contributor.author |
Hemara Lauren M |
|
dc.contributor.author |
McCann Honour C |
|
dc.contributor.author |
Yoon Minsoo |
|
dc.contributor.author |
Warring Suzanne L |
|
dc.contributor.author |
Fineran Peter C |
|
dc.contributor.author |
Mesarich Carl H |
|
dc.contributor.author |
Templeton Matthew D |
|
dc.date.accessioned |
2020-11-23T21:41:53Z |
|
dc.date.available |
2020-11-23T21:41:53Z |
|
dc.date.issued |
2020-9-28 |
|
dc.identifier.citation |
Environmental microbiology 28 Sep 2020 |
|
dc.identifier.issn |
1462-2912 |
|
dc.identifier.uri |
http://hdl.handle.net/2292/53671 |
|
dc.description.abstract |
The common polysaccharide antigen (CPA) of the lipopolysaccharide (LPS) from Pseudomonas syringae is highly variable, but the genetic basis for this is poorly understood. We have characterised the CPA locus from P. syringae pv. actinidiae (Psa). This locus has genes for L- and D-rhamnose biosynthesis and an operon coding for ABC transporter subunits, a bifunctional glycosyltransferase and an O-methyltransferase. This operon is predicted to have a role in the transport, elongation and termination of the CPA oligosaccharide and is referred to as the TET operon. Two alleles of the TET operon were present in different biovars (BV) of Psa and lineages of the closely related pathovar P. syringae pv. actinidifoliorum. This allelic variation was reflected in the electrophoretic properties of purified LPS from the different isolates. Gene knockout of the TET operon allele from BV1 and replacement with that from BV3, demonstrated the link between the genetic locus and the biochemical properties of the LPS molecules in Psa. Sequence analysis of the TET operon from a range of P. syringae and P. viridiflava isolates displayed a phylogenetic history incongruent with core gene phylogeny, but correlates with previously reported tailocin sensitivity, suggesting a functional relationship between LPS structure and tailocin susceptibility. This article is protected by copyright. All rights reserved. |
|
dc.format.medium |
Print-Electronic |
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dc.language |
eng |
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dc.relation.ispartofseries |
Environmental microbiology |
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dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc/4.0/ |
|
dc.subject |
0605 Microbiology |
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dc.title |
Variation at the common polysaccharide antigen locus drives lipopolysaccharide diversity within the Pseudomonas syringae species complex. |
|
dc.type |
Journal Article |
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dc.identifier.doi |
10.1111/1462-2920.15250 |
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dc.date.updated |
2020-10-11T23:20:30Z |
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dc.rights.holder |
Copyright: The authors |
en |
pubs.publication-status |
Published |
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dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Journal Article |
|
pubs.elements-id |
817176 |
|
dc.identifier.eissn |
1462-2920 |
|