dc.contributor.author |
Soh, Kar Yan |
|
dc.contributor.author |
Loh, Jacelyn Mei San |
|
dc.contributor.author |
Hall, Christopher |
|
dc.contributor.author |
Proft, Thomas |
|
dc.coverage.spatial |
Queenstown |
|
dc.date.accessioned |
2020-12-08T21:25:47Z |
|
dc.date.available |
2020-12-08T21:25:47Z |
|
dc.date.issued |
2020-9-5 |
|
dc.identifier.citation |
Microorganisms 8(9) 05 Sep 2020 |
|
dc.identifier.issn |
2076-2607 |
|
dc.identifier.uri |
http://hdl.handle.net/2292/53862 |
|
dc.description.abstract |
Streptococcus iniae is a major fish pathogen that contributes to large annual losses in the aquaculture industry, exceeding US$100 million. It is also reported to cause opportunistic infections in humans. We have recently identified two novel S. iniae virulence factors, an extracellular nuclease (SpnAi) and a secreted nucleotidase (S5nAi), and verified their predicted enzymatic activities using recombinant proteins. Here, we report the generation of green fluorescent S. iniae spnAi and s5nAi deletion mutants and their evaluation in a transgenic zebrafish infection model. Our results show nuclease and nucleotidase activities in S. iniae could be attributed to SpnAi and S5nAi, respectively. Consistent with this, larvae infected with the deletion mutants demonstrated enhanced survival and bacterial clearance, compared to those infected with wild-type (WT) S. iniae. Deletion of spnAi and s5nAi resulted in sustained recruitment of neutrophils and macrophages, respectively, to the site of infection. We also show that recombinant SpnAi is able to degrade neutrophil extracellular traps (NETs) isolated from zebrafish kidney tissue. Our results suggest that both enzymes play an important role in S. iniae immune evasion and might present potential targets for the development of therapeutic agents or vaccines. |
|
dc.format.medium |
Electronic |
|
dc.language |
eng |
|
dc.publisher |
MDPI AG |
|
dc.relation.ispartof |
Australasian Society of Immunology (ASI) satellite |
|
dc.relation.ispartof |
QMB conference |
|
dc.relation.ispartofseries |
Microorganisms |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Microbiology |
|
dc.subject |
nuclease |
|
dc.subject |
SpnAi |
|
dc.subject |
nucleotidase |
|
dc.subject |
S5nAi |
|
dc.subject |
Streptococcus iniae |
|
dc.subject |
virulence |
|
dc.subject |
zebrafish infection model |
|
dc.subject |
immune evasion |
|
dc.subject |
NEUTROPHIL EXTRACELLULAR TRAPS |
|
dc.subject |
IMMUNE-SYSTEM |
|
dc.subject |
ADENOSINE |
|
dc.subject |
EXPRESSION |
|
dc.subject |
PATHOGEN |
|
dc.subject |
MACROPHAGES |
|
dc.subject |
NUCLEASE |
|
dc.subject |
CAPSULE |
|
dc.subject |
ESCAPE |
|
dc.subject |
IDENTIFICATION |
|
dc.title |
Functional Analysis of Two Novel Streptococcus iniae Virulence Factors Using a Zebrafish Infection Model. |
|
dc.type |
Presentation |
|
dc.identifier.doi |
10.3390/microorganisms8091361 |
|
pubs.issue |
9 |
|
pubs.begin-page |
1361 |
|
pubs.volume |
8 |
|
dc.date.updated |
2020-11-16T22:45:52Z |
|
dc.rights.holder |
Copyright: The authors |
en |
pubs.author-url |
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000582036600001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e41486220adb198d0efde5a3b153e7d |
|
pubs.finish-date |
2018-8-27 |
|
pubs.publication-status |
Published |
|
pubs.start-date |
2018-8-26 |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
research-article |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
758312 |
|
dc.identifier.eissn |
2076-2607 |
|