dc.contributor.author |
Oh, Jin Kyo |
|
dc.contributor.author |
Przepiorski, Aneta |
|
dc.contributor.author |
Chang, Hao-Han |
|
dc.contributor.author |
Dodd, Rachel C |
|
dc.contributor.author |
Sander, Veronika |
|
dc.contributor.author |
Sorrenson, Brie |
|
dc.contributor.author |
Shih, Jen-Hsing |
|
dc.contributor.author |
Hollywood, Jennifer A |
|
dc.contributor.author |
de Zoysa, Janak R |
|
dc.contributor.author |
Shepherd, Peter R |
|
dc.contributor.author |
Davidson, Alan J |
|
dc.contributor.author |
Holm, Teresa M |
|
dc.date.accessioned |
2020-12-08T23:01:16Z |
|
dc.date.available |
2020-12-08T23:01:16Z |
|
dc.date.issued |
2020-10-18 |
|
dc.identifier.issn |
0303-6758 |
|
dc.identifier.uri |
http://hdl.handle.net/2292/53895 |
|
dc.description.abstract |
© 2020 The Royal Society of New Zealand. We aimed to generate human induced pluripotent stem cell (iPSC) lines from New Zealand donors. These lines are the first to be generated in New Zealand. Human dermal fibroblasts were collected from two individual donors and reprogrammed with the human OSKM transcription factors using the Sendai virus system. Emerging iPSC colonies were picked, expanded and karyotyped. Clones with normal karyotype were characterised for pluripotency marker expression, p53 mutational status and trilineage differentiation potential. The MANZ-2-2 and MANZ-4-37 iPSC lines showed normal karyotype and expressed pluripotency markers at RNA and protein levels without detectable transgene expression. Both lines differentiated into the three germ layers in vitro and passed the hPSC Scorecard assay for pluripotency and trilineage differentiation. Furthermore, both lines were susceptible to cell apoptosis mediated by nutlin-3a indicative of their wildtype p53 status. This study presents the successful derivation and characterisation of iPSC lines derived from New Zealand donors. These lines will facilitate iPSC-based research in New Zealand and beyond. |
|
dc.language |
en |
|
dc.publisher |
Informa UK Limited |
|
dc.relation.ispartofseries |
Journal of the Royal Society of New Zealand |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.subject |
Science & Technology |
|
dc.subject |
Multidisciplinary Sciences |
|
dc.subject |
Science & Technology - Other Topics |
|
dc.subject |
Cell reprogramming |
|
dc.subject |
induced pluripotent stem cells |
|
dc.subject |
pluripotency |
|
dc.subject |
differentiation |
|
dc.subject |
nutlin-3a assay |
|
dc.subject |
BETA-CELLS |
|
dc.subject |
GENERATION |
|
dc.title |
Derivation of induced pluripotent stem cell lines from New Zealand donors |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1080/03036758.2020.1830808 |
|
pubs.begin-page |
1 |
|
dc.date.updated |
2020-11-06T08:22:21Z |
|
dc.rights.holder |
Copyright: The author |
en |
pubs.author-url |
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000579193700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e41486220adb198d0efde5a3b153e7d |
|
pubs.end-page |
14 |
|
pubs.publication-status |
Published online |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
|
pubs.subtype |
Early Access |
|
pubs.subtype |
Journal |
|
pubs.elements-id |
822899 |
|
dc.identifier.eissn |
1175-8899 |
|
pubs.online-publication-date |
2020-10-18 |
|