Derivation of induced pluripotent stem cell lines from New Zealand donors

Show simple item record Oh, Jin Kyo Przepiorski, Aneta Chang, Hao-Han Dodd, Rachel C Sander, Veronika Sorrenson, Brie Shih, Jen-Hsing Hollywood, Jennifer A de Zoysa, Janak R Shepherd, Peter R Davidson, Alan J Holm, Teresa M 2020-12-08T23:01:16Z 2020-12-08T23:01:16Z 2020-10-18
dc.identifier.issn 0303-6758
dc.description.abstract © 2020 The Royal Society of New Zealand. We aimed to generate human induced pluripotent stem cell (iPSC) lines from New Zealand donors. These lines are the first to be generated in New Zealand. Human dermal fibroblasts were collected from two individual donors and reprogrammed with the human OSKM transcription factors using the Sendai virus system. Emerging iPSC colonies were picked, expanded and karyotyped. Clones with normal karyotype were characterised for pluripotency marker expression, p53 mutational status and trilineage differentiation potential. The MANZ-2-2 and MANZ-4-37 iPSC lines showed normal karyotype and expressed pluripotency markers at RNA and protein levels without detectable transgene expression. Both lines differentiated into the three germ layers in vitro and passed the hPSC Scorecard assay for pluripotency and trilineage differentiation. Furthermore, both lines were susceptible to cell apoptosis mediated by nutlin-3a indicative of their wildtype p53 status. This study presents the successful derivation and characterisation of iPSC lines derived from New Zealand donors. These lines will facilitate iPSC-based research in New Zealand and beyond.
dc.language en
dc.publisher Informa UK Limited
dc.relation.ispartofseries Journal of the Royal Society of New Zealand
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.subject Science & Technology
dc.subject Multidisciplinary Sciences
dc.subject Science & Technology - Other Topics
dc.subject Cell reprogramming
dc.subject induced pluripotent stem cells
dc.subject pluripotency
dc.subject differentiation
dc.subject nutlin-3a assay
dc.subject BETA-CELLS
dc.subject GENERATION
dc.title Derivation of induced pluripotent stem cell lines from New Zealand donors
dc.type Journal Article
dc.identifier.doi 10.1080/03036758.2020.1830808
pubs.begin-page 1 2020-11-06T08:22:21Z
dc.rights.holder Copyright: The author en
pubs.end-page 14
pubs.publication-status Published online
dc.rights.accessrights en
pubs.subtype Article
pubs.subtype Early Access
pubs.subtype Journal
pubs.elements-id 822899
dc.identifier.eissn 1175-8899 2020-10-18

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