The protozoan Trichomonas vaginalis targets bacteria with laterally-acquired NlpC/P60 peptidoglycan hydrolases

Show simple item record

dc.contributor.author Pinheiro, Jully
dc.contributor.author Biboy, Jacob
dc.contributor.author Vollmer, Waldemar
dc.contributor.author Hirt, Robert P
dc.contributor.author Keown, Jeremy R
dc.contributor.author Artuyants, Anastasiia
dc.contributor.author Goldstone, David C
dc.contributor.author Simoes-Barbosa, Augusto
dc.date.accessioned 2020-12-09T01:10:10Z
dc.date.available 2020-12-09T01:10:10Z
dc.identifier.citation BioRxiv (10.1101/320382). 11 May 2018
dc.identifier.uri http://hdl.handle.net/2292/53962
dc.description.abstract <jats:title>Abstract</jats:title><jats:p><jats:italic>Trichomonas vaginalis</jats:italic>is a human eukaryotic pathogen and the causative agent of trichomoniasis, the most prevalent non-viral sexually transmitted infection worldwide. This extracellular protozoan parasite is intimately associated with the human vaginal mucosa and microbiota but key aspects of the complex interactions between the parasite and the vaginal bacteria remain elusive. We report that<jats:italic>T. vaginalis</jats:italic>has acquired, by lateral gene transfer from bacteria, genes encoding peptidoglycan hydrolases of the NlpC/P60 family. Two of the<jats:italic>T. vaginalis</jats:italic>enzymes were active against bacterial peptidoglycan, retaining the active site fold and specificity as DL-endopeptidases. The endogenous NlpC/P60 genes are transcriptionally up regulated in<jats:italic>T. vaginalis</jats:italic>when in the presence of bacteria. The over-expression of an exogenous copy produces a remarkable phenotype where the parasite is capable of competing out bacteria from mixed cultures, consistent with the biochemical activity of the enzyme<jats:italic>in vitro</jats:italic>. Our study highlights the relevance of the interactions of this eukaryotic pathogen with bacteria, a poorly understood aspect on the biology of this important human parasite.</jats:p><jats:sec><jats:title>Author summary</jats:title><jats:p><jats:italic>Trichomonas vaginalis</jats:italic>is a protozoan parasite that causes a very common sexually transmitted disease known as trichomoniasis. This extracellular parasite resides in the vagina where it is in close association with the mucosa and the local microbiota. Very little is known about the nature of the parasite-bacteria interactions. Here, we report that this parasite had acquired genes from bacteria which retained their original function producing active enzymes capable of degrading peptidoglycan, a polymer that is chemically unique to the cell envelope of bacteria. Our results indicate that these enzymes help the parasite compete out bacteria in mixed cultures. These observations suggest that these enzymes may be critical for the parasite to establish infection in the vagina, a body site that is densely colonised with bacteria. Our study further highlights the importance of understanding the interactions between pathogens and microbiota, as the outcomes of these interactions are increasingly understood to have important implications on health and disease.</jats:p></jats:sec>
dc.publisher Cold Spring Harbor Laboratory
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.title The protozoan Trichomonas vaginalis targets bacteria with laterally-acquired NlpC/P60 peptidoglycan hydrolases
dc.type Journal Article
dc.identifier.doi 10.1101/320382
dc.date.updated 2020-11-16T17:17:38Z
dc.rights.holder Copyright: The author en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.elements-id 825793


Files in this item

Find Full text

This item appears in the following Collection(s)

Show simple item record

Share

Search ResearchSpace


Browse

Statistics