dc.contributor.author |
Deng, Cecilia H |
|
dc.contributor.author |
Plummer, Kim M |
|
dc.contributor.author |
Jones, Darcy AB |
|
dc.contributor.author |
Mesarich, Carl H |
|
dc.contributor.author |
Shiller, Jason |
|
dc.contributor.author |
Taranto, Adam P |
|
dc.contributor.author |
Robinson, Andrew J |
|
dc.contributor.author |
Kastner, Patrick |
|
dc.contributor.author |
Hall, Nathan E |
|
dc.contributor.author |
Templeton, Matthew D |
|
dc.contributor.author |
Bowen, Joanna K |
|
dc.coverage.spatial |
England |
|
dc.date.accessioned |
2021-01-13T21:49:39Z |
|
dc.date.available |
2021-01-13T21:49:39Z |
|
dc.date.issued |
2017-5-2 |
|
dc.identifier.citation |
BMC genomics 18(1):339 02 May 2017 |
|
dc.identifier.issn |
1471-2164 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/54219 |
|
dc.description.abstract |
Fungal plant pathogens belonging to the genus Venturia cause damaging scab diseases of members of the Rosaceae. In terms of economic impact, the most important of these are V. inaequalis, which infects apple, and V. pirina, which is a pathogen of European pear. Given that Venturia fungi colonise the sub-cuticular space without penetrating plant cells, it is assumed that effectors that contribute to virulence and determination of host range will be secreted into this plant-pathogen interface. Thus the predicted secretomes of a range of isolates of Venturia with distinct host-ranges were interrogated to reveal putative proteins involved in virulence and pathogenicity.Genomes of Venturia pirina (one European pear scab isolate) and Venturia inaequalis (three apple scab, and one loquat scab, isolates) were sequenced and the predicted secretomes of each isolate identified. RNA-Seq was conducted on the apple-specific V. inaequalis isolate Vi1 (in vitro and infected apple leaves) to highlight virulence and pathogenicity components of the secretome. Genes encoding over 600 small secreted proteins (candidate effectors) were identified, most of which are novel to Venturia, with expansion of putative effector families a feature of the genus. Numerous genes with similarity to Leptosphaeria maculans AvrLm6 and the Verticillium spp. Ave1 were identified. Candidates for avirulence effectors with cognate resistance genes involved in race-cultivar specificity were identified, as were putative proteins involved in host-species determination. Candidate effectors were found, on average, to be in regions of relatively low gene-density and in closer proximity to repeats (e.g. transposable elements), compared with core eukaryotic genes.Comparative secretomics has revealed candidate effectors from Venturia fungal plant pathogens that attack pome fruit. Effectors that are putative determinants of host range were identified; both those that may be involved in race-cultivar and host-species specificity. Since many of the effector candidates are in close proximity to repetitive sequences this may point to a possible mechanism for the effector gene family expansion observed and a route to diversification via transposition and repeat-induced point mutation. |
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dc.format.medium |
Electronic |
|
dc.language |
eng |
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dc.publisher |
Springer Science and Business Media LLC |
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dc.relation.ispartofseries |
BMC genomics |
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dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
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dc.subject |
Cell Wall |
|
dc.subject |
Ascomycota |
|
dc.subject |
Rosaceae |
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dc.subject |
Genomics |
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dc.subject |
Virulence |
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dc.subject |
Plant Diseases |
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dc.subject |
Host Specificity |
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dc.subject |
Apple |
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dc.subject |
Effector |
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dc.subject |
European pear |
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dc.subject |
Malus x domestica |
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dc.subject |
Pyrus communis |
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dc.subject |
Secretome |
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dc.subject |
Venturia inaequalis |
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dc.subject |
Venturia pirina |
|
dc.subject |
Ascomycota |
|
dc.subject |
Cell Wall |
|
dc.subject |
Genomics |
|
dc.subject |
Host Specificity |
|
dc.subject |
Plant Diseases |
|
dc.subject |
Rosaceae |
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dc.subject |
Virulence |
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dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Biotechnology & Applied Microbiology |
|
dc.subject |
Genetics & Heredity |
|
dc.subject |
Venturia inaequalis |
|
dc.subject |
Venturia pirina |
|
dc.subject |
Apple |
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dc.subject |
Malus x domestica |
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dc.subject |
European pear |
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dc.subject |
Pyrus communis |
|
dc.subject |
Secretome |
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dc.subject |
Effector |
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dc.subject |
MYCOSPHAERELLA-GRAMINICOLA REVEALS |
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dc.subject |
FILAMENTOUS PLANT-PATHOGENS |
|
dc.subject |
CARBOHYDRATE-ACTIVE ENZYMES |
|
dc.subject |
RNA-SEQ DATA |
|
dc.subject |
CELL-WALL |
|
dc.subject |
PROTEIN FAMILIES |
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dc.subject |
GENE FAMILY |
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dc.subject |
FUNCTIONAL-ANALYSIS |
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dc.subject |
AVIRULENCE GENES |
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dc.subject |
HYDROPHOBIN GENE |
|
dc.subject |
Apple |
|
dc.subject |
Effector |
|
dc.subject |
European pear |
|
dc.subject |
Malus x domestica |
|
dc.subject |
Pyrus communis |
|
dc.subject |
Secretome |
|
dc.subject |
Venturia inaequalis |
|
dc.subject |
Venturia pirina |
|
dc.subject |
Ascomycota |
|
dc.subject |
Cell Wall |
|
dc.subject |
Genomics |
|
dc.subject |
Host Specificity |
|
dc.subject |
Plant Diseases |
|
dc.subject |
Rosaceae |
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dc.subject |
Virulence |
|
dc.subject |
0604 Genetics |
|
dc.subject |
0607 Plant Biology |
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dc.subject |
1108 Medical Microbiology |
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dc.subject |
0605 Microbiology |
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dc.subject |
Basic Science |
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dc.subject |
Infectious Diseases |
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dc.subject |
Vaccine Related |
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dc.subject |
Immunization |
|
dc.subject |
Genetics |
|
dc.subject |
Infection |
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dc.subject |
2.2 Factors relating to physical environment |
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dc.subject |
06 Biological Sciences |
|
dc.subject |
08 Information and Computing Sciences |
|
dc.subject |
11 Medical and Health Sciences |
|
dc.title |
Comparative analysis of the predicted secretomes of Rosaceae scab pathogens Venturia inaequalis and V. pirina reveals expanded effector families and putative determinants of host range. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1186/s12864-017-3699-1 |
|
pubs.issue |
1 |
|
pubs.begin-page |
339 |
|
pubs.volume |
18 |
|
dc.date.updated |
2020-12-02T03:39:23Z |
|
dc.rights.holder |
Copyright: The authors |
en |
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/28464870 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Comparative Study |
|
pubs.subtype |
Research Support, Non-U.S. Gov't |
|
pubs.subtype |
research-article |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
626734 |
|
dc.identifier.eissn |
1471-2164 |
|
dc.identifier.pii |
10.1186/s12864-017-3699-1 |
|
pubs.number |
339 |
|
pubs.online-publication-date |
2017-5-2 |
|