Abstract:
One of the most effective treatments for Limbal Stem Cells Deficiency (LSCD) is Cultivated Limbal Epithelial Stem Cells (CLET). Precise knowledge of limbal stem cells culture increase the quality of treatment, mitigate the side effects and complications of organ transplantation, and expedite the treatment process. However, protocols for CLET need further optimization and standardization. This mini review covers basic concepts and important criteria that should be taken into consideration in designing a successful CLET that can be helpful for other targets of regenerative medicine.