Factors Associated With the Microbiome in Moderate–Late Preterm Babies: A Cohort Study From the DIAMOND Randomized Controlled Trial

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dc.contributor.author Chong, Clara Yieh Lin
dc.contributor.author Vatanen, Tommi
dc.contributor.author Alexander, Tanith
dc.contributor.author Bloomfield, Frank H
dc.contributor.author O’Sullivan, Justin M
dc.date.accessioned 2021-04-16T00:32:01Z
dc.date.available 2021-04-16T00:32:01Z
dc.date.issued 2021-3-1
dc.identifier.citation Frontiers in Cellular and Infection Microbiology 11 01 Mar 2021
dc.identifier.issn 2235-2988
dc.identifier.uri https://hdl.handle.net/2292/54905
dc.description.abstract The gut microbiota of preterm infants is affected by perinatal factors and, in turn, may impact upon infant health. In this study, we collected fecal samples at Day-10 (D10) and 4-months corrected-age (4M) from 227 moderate-late preterm (MLPT) babies enrolled in a randomized controlled trial of nutritional management. A total of 320 samples underwent 16S amplicon sequencing, and shotgun metagenomic sequencing was performed on 94 samples from the 4M time point. The microbiome of babies whose families lived in lower socioeconomic status (SES) areas exhibited a significantly higher microbial alpha diversity at D10 (Wilcoxon test<i>, p</i> = 0.021), greater abundance of <i>Bifidobacterium</i> (linear model, q = 0.020) at D10 and <i>Megasphaera</i> (q = 0.031) at 4M. Hospital of birth explained 5.2% of the observed variance in 4M samples (PERMANOVA, <i>p</i> = 0.038), with <i>Staphylococcus aureus</i> more abundant in fecal samples from babies born in Middlemore hospital (linear model, q = 0.016). Maternal antibiotic (Wilcoxon test, <i>p</i> = 0.013) and probiotic (<i>p</i> = 0.04) usage within the four-week period before sample collection was associated with a reduction in the alpha diversity of D10 samples. Infant probiotic intake explained 2.1% (PERMANOVA, <i>p</i> = 0.021) of the variance in the D10 microbial profile with increased <i>Lactobacillus</i> (linear model, q = 1.1 × 10<sup>-10</sup>) levels. At 4M, the microbiome of infants who were breastmilk fed had reduced alpha diversity when compared to non-breastmilk fed infants (Wilcoxon test, <i>p</i> < 0.05). Although causality cannot be inferred within our study, we conclude that in MLPT babies, maternal socioeconomic factors, as well as the perinatal medical environment and nutrition impact on the development of the newborn microbiome.
dc.publisher Frontiers Media SA
dc.relation.ispartofseries Frontiers in Cellular and Infection Microbiology
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm
dc.rights.uri https://www.frontiersin.org/about/policies-and-publication-ethics
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject 0601 Biochemistry and Cell Biology
dc.subject 0605 Microbiology
dc.title Factors Associated With the Microbiome in Moderate–Late Preterm Babies: A Cohort Study From the DIAMOND Randomized Controlled Trial
dc.type Journal Article
dc.identifier.doi 10.3389/fcimb.2021.595323
pubs.begin-page 595323
pubs.volume 11
dc.date.updated 2021-03-25T18:56:33Z
dc.rights.holder Copyright: The author en
pubs.publication-status Published online
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Journal Article
pubs.elements-id 842108
dc.identifier.eissn 2235-2988
pubs.online-publication-date 2021-3-1

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