dc.contributor.author |
Thomson, Geoffrey |
|
dc.contributor.author |
Zhang, Lulu |
|
dc.contributor.author |
Wen, Jiangqi |
|
dc.contributor.author |
Mysore, Kirankumar S |
|
dc.contributor.author |
Putterill, Joanna |
|
dc.coverage.spatial |
Switzerland |
|
dc.date.accessioned |
2021-05-12T00:18:31Z |
|
dc.date.available |
2021-05-12T00:18:31Z |
|
dc.date.issued |
2021-1 |
|
dc.identifier.citation |
Frontiers in plant science 12:634091 Jan 2021 |
|
dc.identifier.issn |
1664-462X |
|
dc.identifier.uri |
https://hdl.handle.net/2292/55083 |
|
dc.description.abstract |
Flowering time influences the yield and productivity of legume crops. <i>Medicago truncatula</i> is a reference temperate legume that, like the winter annual <i>Arabidopsis thaliana</i>, shows accelerated flowering in response to vernalization (extended cold) and long-day (LD) photoperiods (VLD). However, unlike <i>A. thaliana</i>, <i>M. truncatula</i> appears to lack functional homologs of core flowering time regulators <i>CONSTANS</i> (<i>CO</i>) and <i>FLOWERING LOCUS C</i> (<i>FLC</i>) which act upstream of the mobile florigen FLOWERING LOCUS T (FT). <i>Medicago truncatula</i> has three LD-induced <i>FT-like</i> genes (<i>MtFTa1</i>, <i>MtFTb1</i>, and <i>MtFTb2</i>) with <i>MtFTa1</i> promoting <i>M. truncatula</i> flowering in response to VLD. Another photoperiodic regulator in <i>A. thaliana</i>, <i>FE</i>, acts to induce <i>FT</i> expression. It also regulates the FT transport pathway and is required for phloem development. Our study identifies a <i>M. truncatula FE</i> homolog <i>Medtr6g444980</i> (<i>MtFE</i>) which complements the late flowering <i>fe-1</i> mutant when expressed from the phloem-specific <i>SUCROSE-PROTON SYMPORTER 2</i> (<i>SUC2</i>) promoter. Analysis of two <i>M. truncatula Tnt1</i> insertional mutants indicate that <i>MtFE</i> promotes flowering in LD and VLD and growth in all conditions tested. Expression of <i>MtFTa1</i>, <i>MtFTb1</i>, and <i>MtFTb2</i> are reduced in <i>Mtfe</i> mutant (NF5076), correlating with its delayed flowering. The NF5076 mutant plants are much smaller than wild type indicating that <i>MtFE</i> is important for normal plant growth. The second mutant (NF18291) displays seedling lethality, like strong <i>fe</i> mutants. We searched for mutants in <i>MtFTb1</i> and <i>MtFTb2</i> identifying a <i>Mtftb2</i> knock out <i>Tnt1</i> mutant (NF20803). However, it did not flower significantly later than wild type. Previously, yeast-two-hybrid assays (Y2H) suggested that <i>Arabidopsis</i> FE interacted with CO and NUCLEAR FACTOR-Y (NF-Y)-like proteins to regulate <i>FT</i>. We found that MtFE interacts with CO and also <i>M. truncatula</i> NF-Y-like proteins in Y2H experiments. Our study indicates that despite the apparent absence of a functional <i>MtCO-like</i> gene, <i>M. truncatula FE</i> likely influences photoperiodic <i>FT</i> expression and flowering time in <i>M. truncatula via</i> a partially conserved mechanism with <i>A. thaliana</i>. |
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dc.format.medium |
Electronic-eCollection |
|
dc.language |
eng |
|
dc.publisher |
Frontiers Media SA |
|
dc.relation.ispartofseries |
Frontiers in plant science |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
Arabidopsis thaliana |
|
dc.subject |
CONSTANS |
|
dc.subject |
Medicago truncatula |
|
dc.subject |
MtFE |
|
dc.subject |
MtFTa1 |
|
dc.subject |
MtFTb |
|
dc.subject |
NUCLEAR FACTOR-Y |
|
dc.subject |
photoperiodic flowering time |
|
dc.subject |
0607 Plant Biology |
|
dc.title |
The Candidate Photoperiod Gene <i>MtFE</i> Promotes Growth and Flowering in <i>Medicago truncatula</i>. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.3389/fpls.2021.634091 |
|
pubs.begin-page |
634091 |
|
pubs.volume |
12 |
|
dc.date.updated |
2021-04-18T21:33:39Z |
|
dc.rights.holder |
Copyright: The authors |
en |
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/33841463 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
research-article |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
847479 |
|
dc.identifier.eissn |
1664-462X |
|
pubs.online-publication-date |
2021-3-26 |
|