dc.contributor.author |
Sharma, Nabangshu |
|
dc.contributor.author |
Langley, Ries J |
|
dc.contributor.author |
Eurtivong, Chatchakorn |
|
dc.contributor.author |
Leung, Euphemia |
|
dc.contributor.author |
Dixon, Ryan Joseph |
|
dc.contributor.author |
Paulin, Emily K |
|
dc.contributor.author |
Rees, Shaun WP |
|
dc.contributor.author |
Pilkington, Lisa I |
|
dc.contributor.author |
Barker, David |
|
dc.contributor.author |
Reynisson, Jóhannes |
|
dc.contributor.author |
Leung, Ivanhoe KH |
|
dc.coverage.spatial |
England |
|
dc.date.accessioned |
2021-07-19T04:13:52Z |
|
dc.date.available |
2021-07-19T04:13:52Z |
|
dc.date.issued |
2021-2 |
|
dc.identifier.issn |
1759-9660 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/55611 |
|
dc.description.abstract |
The Bacillus cereus phosphatidylcholine-specific phospholipase C (PC-PLCBc) is an enzyme that catalyses the hydrolysis of phosphatidylcholines into phosphocholine and 1,2-diacylglycerols. PC-PLCBc has found applications in both the food industry and in medicinal chemistry. Herein, we report our work in the development and optimisation of a matrix assisted laser desorption ionisation time-of-flight (MALDI-TOF) mass spectrometry-based assay to monitor PC-PLCBc activity. The use of one-phase and two-phase reaction systems to assess the inhibition of PC-PLCBc with different structural classes of inhibitors was compared. We also highlighted the advantage of our assay over the commonly used commercially available Amplex Red assay. This method will also be applicable to work on the activity and inhibition of other phospholipases. |
|
dc.format.medium |
Print |
|
dc.language |
eng |
|
dc.publisher |
Royal Society of Chemistry (RSC) |
|
dc.relation.ispartofseries |
Analytical methods : advancing methods and applications |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.subject |
Bacillus cereus |
|
dc.subject |
Phosphatidylcholines |
|
dc.subject |
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
|
dc.subject |
Type C Phospholipases |
|
dc.subject |
Bacillus cereus |
|
dc.subject |
Phosphatidylcholines |
|
dc.subject |
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
|
dc.subject |
Type C Phospholipases |
|
dc.subject |
Science & Technology |
|
dc.subject |
Physical Sciences |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Technology |
|
dc.subject |
Chemistry, Analytical |
|
dc.subject |
Food Science & Technology |
|
dc.subject |
Spectroscopy |
|
dc.subject |
Chemistry |
|
dc.subject |
0301 Analytical Chemistry |
|
dc.subject |
0399 Other Chemical Sciences |
|
dc.title |
An optimised MALDI-TOF assay for phosphatidylcholine-specific phospholipase C. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1039/d0ay02208j |
|
pubs.issue |
4 |
|
pubs.begin-page |
491 |
|
pubs.volume |
13 |
|
dc.date.updated |
2021-06-24T23:12:11Z |
|
dc.rights.holder |
Copyright: The author |
en |
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/33432952 |
|
pubs.end-page |
496 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
833844 |
|
dc.identifier.eissn |
1759-9679 |
|
pubs.online-publication-date |
2021 |
|