dc.contributor.author |
Davidson, Joanne O |
|
dc.contributor.author |
van den Heuij, Lotte G |
|
dc.contributor.author |
Fraser, Mhoyra |
|
dc.contributor.author |
Wassink, Guido |
|
dc.contributor.author |
Miller, Suzanne L |
|
dc.contributor.author |
Lim, Rebecca |
|
dc.contributor.author |
Wallace, Euan M |
|
dc.contributor.author |
Jenkin, Graham |
|
dc.contributor.author |
Gunn, Alistair J |
|
dc.contributor.author |
Bennet, Laura |
|
dc.coverage.spatial |
United States |
|
dc.date.accessioned |
2021-08-05T22:52:21Z |
|
dc.date.available |
2021-08-05T22:52:21Z |
|
dc.date.issued |
2021-3 |
|
dc.identifier.citation |
Stem cells translational medicine 10(3):427-440 Mar 2021 |
|
dc.identifier.issn |
2157-6564 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/55863 |
|
dc.description.abstract |
There is increasing evidence that administration of many types of stem cells, including human amnion epithelial cells (hAECs), can reduce hypoxic-ischemic injury, including in the perinatal brain. However, the therapeutic window for single dose treatment is not known. We compared the effects of early and delayed intracerebroventricular administration of hAECs in fetal sheep at 0.7 gestation on brain injury induced by 25 minutes of complete umbilical cord occlusion (UCO) or sham occlusion. Fetuses received either 1 × 10<sup>6</sup> hAECs or vehicle alone, as an infusion over 1 hour, either 2 or 24 hours after UCO. Fetuses were killed for brain histology at 7 days post-UCO. hAEC infusion at both 2 and 24 hours had dramatic anti-inflammatory and anti-gliotic effects, including significantly attenuating the increase in microglia after UCO in the white and gray matter and the number of astrocytes in the white matter. Both protocols partially improved myelination, but had no effect on total or immature/mature numbers of oligodendrocytes. Neuronal survival in the hippocampus was increased by hAEC infusion at either 2 or 24 hours, whereas only hAECs at 24 hours were associated with improved neuronal survival in the striatum and thalamus. Neither protocol improved recovery of electroencephalographic (EEG) power. These data suggest that a single infusion of hAECs is anti-inflammatory, anti-gliotic, and neuroprotective in preterm fetal sheep when given up to 24 hours after hypoxia-ischemia, but was associated with limited white matter protection after 7 days recovery and no improvement in the recovery of EEG power. |
|
dc.format.medium |
Print-Electronic |
|
dc.language |
eng |
|
dc.publisher |
Wiley |
|
dc.relation.ispartofseries |
Stem cells translational medicine |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc/4.0/ |
|
dc.subject |
asphyxia |
|
dc.subject |
inflammation |
|
dc.subject |
neuroprotection |
|
dc.subject |
preterm birth |
|
dc.subject |
stem cells |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Cell & Tissue Engineering |
|
dc.subject |
Cell Biology |
|
dc.subject |
asphyxia |
|
dc.subject |
inflammation |
|
dc.subject |
neuroprotection |
|
dc.subject |
preterm birth |
|
dc.subject |
stem cells |
|
dc.subject |
BRAIN-INJURY |
|
dc.subject |
CEREBRAL OXYGENATION |
|
dc.subject |
IMPROVES OUTCOMES |
|
dc.subject |
MATTER INJURY |
|
dc.subject |
WHITE |
|
dc.subject |
ISCHEMIA |
|
dc.subject |
ASPHYXIA |
|
dc.subject |
SEIZURES |
|
dc.subject |
THERAPY |
|
dc.subject |
HYPOXIA |
|
dc.subject |
0601 Biochemistry and Cell Biology |
|
dc.subject |
1004 Medical Biotechnology |
|
dc.subject |
1103 Clinical Sciences |
|
dc.title |
Window of opportunity for human amnion epithelial stem cells to attenuate astrogliosis after umbilical cord occlusion in preterm fetal sheep. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1002/sctm.20-0314 |
|
pubs.issue |
3 |
|
pubs.begin-page |
427 |
|
pubs.volume |
10 |
|
dc.date.updated |
2021-07-21T03:55:59Z |
|
dc.rights.holder |
Copyright: The authors |
en |
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/33103374 |
|
pubs.end-page |
440 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
research-article |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
822896 |
|
dc.identifier.eissn |
2157-6580 |
|
pubs.online-publication-date |
2020-10-26 |
|