dc.contributor.author |
Wilson, Mark C. |
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dc.date.accessioned |
2021-08-26T06:17:18Z |
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dc.date.available |
2021-08-26T06:17:18Z |
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dc.date.issued |
1985 |
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dc.identifier.uri |
https://hdl.handle.net/2292/56279 |
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dc.description |
Full text is available to authenticated members of The University of Auckland only. |
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dc.description.abstract |
Previous light-scattering studies of spermatozoon motility have been hampered by the large, asymmetric shape of spermatozoa, which causes difficulty in the interpretation of intensity fluctuations in the light scattered from a single laser beam. This thesis describes an experimental arrangement for measuring the distribution of transit times for swimming spermatozoa using two slightly separated, focused laser beams. The theory of operation of the instrument, the dual-beam laser transit velocimeter, is developed to enable the analysis of the experimentally obtained cross-correlation functions. The effects of the pronounced spermatozoon asymmetry and associated intensity modulation in the scattered light are also investigated, both theoretically and experimentally. The effect of the pronounced orientational dependence of the scattered light intensity on the dual-beam technique is shown to be negligible provided the swimming speed distribution has a coefficient of variation (?f/v) > 0.1. Various experimental configurations of the dual-beam laser transit velocimeter were evaluated and it was found that a 2-colour configuration with a ratio of beam radius to separation of 0.25 was best suited to the study of bull and ram spermatozoa. It is also shown that Number Fluctuation Spectroscopy can be conducted simultaneously with dual-beam velocimetry by utilizing the scattered light signal and that this provides a means of measuring both the rotational and translational speeds of bull spermatozoa. Simultaneous measurements of the rotational and translational speeds of bull and ram spermatozoa are presented and it is demonstrated in a large number of field trials that such measurements provide a sensitive and useful measure of sperm motility. These trials investigate the effects of pH, temperature, aging, oxygen, washing, seminal plasma and dilution on the motility of bull and ram spermatozoa. |
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dc.publisher |
ResearchSpace@Auckland |
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dc.relation.isreferencedby |
UoA999934814002091 |
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dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. |
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dc.rights |
Restricted Item. Full text is available to authenticated members of The University of Auckland only. |
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dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
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dc.title |
Dual beam laser velocimetry of spermatozoa |
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dc.type |
Thesis |
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thesis.degree.discipline |
Physics |
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thesis.degree.grantor |
The University of Auckland |
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thesis.degree.name |
PhD |
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dc.rights.holder |
Copyright: The author |
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dc.identifier.wikidata |
Q112848986 |
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