dc.contributor.author |
Ogilvie, Olivia |
|
dc.contributor.author |
Larsen, Nigel |
|
dc.contributor.author |
Sutton, Kevin |
|
dc.contributor.author |
Domigan, Laura |
|
dc.contributor.author |
Gerrard, Juliet |
|
dc.contributor.author |
Demarais, Nicholas |
|
dc.contributor.author |
Roberts, Sarah |
|
dc.coverage.spatial |
Netherlands |
|
dc.date.accessioned |
2021-09-26T23:10:39Z |
|
dc.date.available |
2021-09-26T23:10:39Z |
|
dc.date.issued |
2020-1 |
|
dc.identifier.citation |
MethodsX 7:101076 Jan 2020 |
|
dc.identifier.citation |
Learning and Instruction 75:101483 01 Oct 2021 |
|
dc.identifier.issn |
2215-0161 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/56673 |
|
dc.description.abstract |
Mass spectrometry (MS) is an emerging method to determine the accurate concentration of immunogenic gluten peptides. It is of interest to quantify specific peptides within the gluten peptidome due to the role they play in the activation of the celiac immune cascade. Celiac disease is an autoimmune disorder triggered in genetically susceptible individuals by the presence of specific gluten peptides that resist digestion in the gastrointestinal tract. The protocol detailed within this paper can accurately quantify (label-free) the concentration of six immunogenic gluten peptides (including the 33mer) released from a food matrix using the INFOGEST <i>in vitro</i> digestion protocol. This method can be used to monitor small changes in the concentration of these marker peptides in response to exogenous factors such as plant-breeding, fermentation or food processing. |
|
dc.format.medium |
Electronic-eCollection |
|
dc.language |
eng |
|
dc.publisher |
Elsevier BV |
|
dc.relation.ispartofseries |
MethodsX |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
dc.subject |
Bread |
|
dc.subject |
Celiac disease |
|
dc.subject |
In vitro digestion |
|
dc.subject |
Peptidomics |
|
dc.subject |
Wheat |
|
dc.subject |
Science & Technology |
|
dc.subject |
Multidisciplinary Sciences |
|
dc.subject |
Science & Technology - Other Topics |
|
dc.subject |
Peptidomics |
|
dc.subject |
Celiac disease |
|
dc.subject |
Bread |
|
dc.subject |
In vitro digestion |
|
dc.subject |
Wheat |
|
dc.subject |
CELIAC-DISEASE |
|
dc.subject |
0912 Materials Engineering |
|
dc.title |
A targeted mass spectrometry method for the accurate label-free quantification of immunogenic gluten peptides produced during simulated digestion of food matrices. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1016/j.mex.2020.101076 |
|
pubs.begin-page |
101076 |
|
pubs.volume |
7 |
|
dc.date.updated |
2021-08-05T00:07:37Z |
|
dc.rights.holder |
Copyright: The author |
en |
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/33033691 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
research-article |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
820114 |
|
dc.identifier.eissn |
2215-0161 |
|
dc.identifier.pii |
S2215-0161(20)30296-X |
|
pubs.number |
101076 |
|