Abstract:
Itaconate is a mammalian antimicrobial metabolite that inhibits the isocitrate lyases (ICLs) of <i>Mycobacterium tuberculosis</i>. Herein, we report that ICLs form a covalent adduct with itaconate through their catalytic cysteine residue. These results reveal atomic details of itaconate inhibition and provide insights into the catalytic mechanism of ICLs.
