dc.contributor.author |
Chen, Yadi |
|
dc.contributor.author |
Gao, Junyuan |
|
dc.contributor.author |
Li, Leping |
|
dc.contributor.author |
Sellitto, Caterina |
|
dc.contributor.author |
Mathias, Richard T |
|
dc.contributor.author |
Donaldson, Paul J |
|
dc.contributor.author |
White, Thomas W |
|
dc.coverage.spatial |
United States |
|
dc.date.accessioned |
2021-10-20T21:10:39Z |
|
dc.date.available |
2021-10-20T21:10:39Z |
|
dc.date.issued |
2019-10 |
|
dc.identifier.citation |
Investigative ophthalmology & visual science 60(13):4416-4424 Oct 2019 |
|
dc.identifier.issn |
0146-0404 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/57058 |
|
dc.description.abstract |
<b>Purpose: </b>Lenses have an intracellular hydrostatic pressure gradient to drive fluid from central fiber cells to surface epithelial cells. Pressure is regulated by a feedback control system that relies on transient receptor potential vanilloid (TRPV)1 and TRPV4 channels. The ciliary muscle transmits tension to the lens through the zonules of Zinn. Here, we have examined if ciliary muscle tension influenced the lens intracellular hydrostatic pressure gradient.<br><br><b>Methods: </b>We measured the ciliary body position and intracellular hydrostatic pressures in mouse lenses while pharmacologically causing relaxation or contraction of the ciliary muscle. We also used inhibitors of TRPV1 and TRPV4, in addition to phosphoinositide 3-kinase (PI3K) p110α knockout mice and immunostaining of phosphorylated protein kinase B (Akt), to determine how changes in ciliary muscle tension resulted in altered hydrostatic pressure.<br><br><b>Results: </b>Ciliary muscle relaxation increased the distance between the ciliary body and the lens and caused a decrease in intracellular hydrostatic pressure that was dependent on intact zonules and could be blocked by inhibition of TRPV4. Ciliary contraction moved the ciliary body toward the lens and caused an increase in intracellular hydrostatic pressure and Akt phosphorylation that required intact zonules and was blocked by either inhibition of TRPV1 or genetic deletion of the p110α catalytic subunit of PI3K.<br><br><b>Conclusions: </b>These results show that the hydrostatic pressure gradient within the lens was influenced by the tension exerted on the lens by the ciliary muscle through the zonules of Zinn. Modulation of the gradient of intracellular hydrostatic pressure in the lens could alter the water content, and the gradient of refractive index. |
|
dc.format.medium |
Print |
|
dc.language |
eng |
|
dc.publisher |
Association for Research in Vision and Ophthalmology (ARVO) |
|
dc.relation.ispartofseries |
Investigative ophthalmology & visual science |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
dc.subject |
Ligaments |
|
dc.subject |
Muscle, Smooth |
|
dc.subject |
Lens, Crystalline |
|
dc.subject |
Ciliary Body |
|
dc.subject |
Animals |
|
dc.subject |
Mice, Inbred C57BL |
|
dc.subject |
Mice, Knockout |
|
dc.subject |
Mice |
|
dc.subject |
Pilocarpine |
|
dc.subject |
Tropicamide |
|
dc.subject |
Mydriatics |
|
dc.subject |
Fluorescent Antibody Technique, Indirect |
|
dc.subject |
Muscle Contraction |
|
dc.subject |
Muscle Relaxation |
|
dc.subject |
Hydrostatic Pressure |
|
dc.subject |
TRPV Cation Channels |
|
dc.subject |
Phosphatidylinositol 3-Kinases |
|
dc.subject |
Animals |
|
dc.subject |
Ciliary Body |
|
dc.subject |
Fluorescent Antibody Technique, Indirect |
|
dc.subject |
Hydrostatic Pressure |
|
dc.subject |
Lens, Crystalline |
|
dc.subject |
Ligaments |
|
dc.subject |
Mice |
|
dc.subject |
Mice, Inbred C57BL |
|
dc.subject |
Mice, Knockout |
|
dc.subject |
Muscle Contraction |
|
dc.subject |
Muscle Relaxation |
|
dc.subject |
Muscle, Smooth |
|
dc.subject |
Mydriatics |
|
dc.subject |
Phosphatidylinositol 3-Kinases |
|
dc.subject |
Pilocarpine |
|
dc.subject |
TRPV Cation Channels |
|
dc.subject |
Tropicamide |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Ophthalmology |
|
dc.subject |
lens epithelium |
|
dc.subject |
membrane channels |
|
dc.subject |
membrane transport |
|
dc.subject |
microcirculation |
|
dc.subject |
SYNERETIC RESPONSE |
|
dc.subject |
OPTICAL-PROPERTIES |
|
dc.subject |
ATP RELEASE |
|
dc.subject |
CIRCULATION |
|
dc.subject |
NA,K-ATPASE |
|
dc.subject |
ACTIVATION |
|
dc.subject |
EPITHELIUM |
|
dc.subject |
EXPRESSION |
|
dc.subject |
AGONISTS |
|
dc.subject |
RABBIT |
|
dc.subject |
1116 Medical Physiology |
|
dc.subject |
Biomedical |
|
dc.subject |
Basic Science |
|
dc.subject |
2.1 Biological and endogenous factors |
|
dc.subject |
06 Biological Sciences |
|
dc.subject |
11 Medical and Health Sciences |
|
dc.title |
The Ciliary Muscle and Zonules of Zinn Modulate Lens Intracellular Hydrostatic Pressure Through Transient Receptor Potential Vanilloid Channels. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1167/iovs.19-27794 |
|
pubs.issue |
13 |
|
pubs.begin-page |
4416 |
|
pubs.volume |
60 |
|
dc.date.updated |
2021-09-21T00:20:56Z |
|
dc.rights.holder |
Copyright: The author |
en |
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/31639828 |
|
pubs.end-page |
4424 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
|
pubs.subtype |
research-article |
|
pubs.subtype |
Journal Article |
|
pubs.subtype |
Research Support, N.I.H., Extramural |
|
pubs.elements-id |
785043 |
|
dc.identifier.eissn |
1552-5783 |
|
dc.identifier.pii |
2753625 |
|