dc.contributor.author |
Yang, Kaiyun |
|
dc.contributor.author |
Tang, Mingtan |
|
dc.contributor.author |
Chang, Hao-Han |
|
dc.contributor.author |
Kanamala, Manju |
|
dc.contributor.author |
Davidson, Alan James |
|
dc.contributor.author |
Wu, Zimei |
|
dc.coverage.spatial |
England |
|
dc.date.accessioned |
2021-11-17T22:23:00Z |
|
dc.date.available |
2021-11-17T22:23:00Z |
|
dc.date.issued |
2021-8-26 |
|
dc.identifier.issn |
1083-7450 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/57480 |
|
dc.description.abstract |
Conventional non-pH-sensitive liposomes for cytoplasmic delivery of protein suffer from poor efficiency. Here we investigated mannosylated pH-sensitive liposomes (MAN-PSL) for cytoplasmic delivery of protein to macrophages RAW 264.7 using PSL and non-pH-sensitive liposomes for comparison. We characterised the pH-dependent fluorescence of green fluorescent protein (GFP) and encapsulated it in liposomes as an intracellular trafficking tracer. GFP showed a reversed 'S'-shaped pH-fluorescence curve with a dramatic signal loss at acidic pH. GFP stored at 4 °C with light protection showed a half-life of 10 days (pH 5-8). The entrapment efficiency of GFP was dominated by the volume ratio of intraliposomal core to external medium for thin-film hydration. Mannosylation did not affect the pH-responsiveness of PSL. Confocal microscopy elucidated that mannosylation promoted the cellular uptake of PSL. For both these liposomes, the strongest, homogeneously distributed GFP fluorescence in the cytoplasm was found at 3 h, confirming efficient endosomal escape of GFP. Conversely, internalisation of non-pH-sensitive liposomes was slow (peaked at 12 h) and both Nile Red and GFP signals remained weak and punctuated in the cytosol. In conclusion, GFP performed as a probe for endosome escape of liposomal cargo. Mannosylation facilitated the internalisation of PSL without compromising their endosomal escape ability. |
|
dc.format.medium |
Print-Electronic |
|
dc.language |
eng |
|
dc.publisher |
Informa UK Limited |
|
dc.relation.ispartofseries |
Pharmaceutical development and technology |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.subject |
Green fluorescent protein |
|
dc.subject |
endosome escape |
|
dc.subject |
entrapment efficiency |
|
dc.subject |
macrophage-targeting |
|
dc.subject |
mannosylated pH-sensitive liposome |
|
dc.subject |
pH-dependent fluorescence |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Pharmacology & Pharmacy |
|
dc.subject |
Green fluorescent protein |
|
dc.subject |
pH-dependent fluorescence |
|
dc.subject |
mannosylated pH-sensitive liposome |
|
dc.subject |
macrophage-targeting |
|
dc.subject |
endosome escape |
|
dc.subject |
entrapment efficiency |
|
dc.subject |
INTRACELLULAR DELIVERY |
|
dc.subject |
CELLULAR TRAFFICKING |
|
dc.subject |
PEGYLATED LIPOSOMES |
|
dc.subject |
IN-VITRO |
|
dc.subject |
INTERNALIZATION |
|
dc.subject |
ENCAPSULATION |
|
dc.subject |
CYTOTOXICITY |
|
dc.subject |
LOCALIZATION |
|
dc.subject |
GEMCITABINE |
|
dc.subject |
MECHANISMS |
|
dc.subject |
1115 Pharmacology and Pharmaceutical Sciences |
|
dc.title |
Mannosylation of pH-sensitive liposomes promoted cytoplasmic delivery of protein to macrophages: green fluorescent protein (GFP) performed as an endosomal escape tracer. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1080/10837450.2021.1969406 |
|
pubs.issue |
9 |
|
pubs.begin-page |
1 |
|
pubs.volume |
26 |
|
dc.date.updated |
2021-10-05T09:07:26Z |
|
dc.rights.holder |
Copyright: The author |
en |
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/34396913 |
|
pubs.end-page |
10 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Journal Article |
|
pubs.elements-id |
863363 |
|
dc.identifier.eissn |
1097-9867 |
|
pubs.online-publication-date |
2021-8-26 |
|