Transcriptomic analysis reveals differences in fruit maturation between two kiwifruit cultivars

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dc.contributor.author Burdon, J
dc.contributor.author Martin, P
dc.contributor.author Ireland, H
dc.contributor.author Schaffer, R
dc.contributor.author McAtee, P
dc.contributor.author Boldingh, H
dc.contributor.author Nardozza, S
dc.date.accessioned 2021-12-09T21:35:08Z
dc.date.available 2021-12-09T21:35:08Z
dc.date.issued 2021-8-1
dc.identifier.issn 0304-4238
dc.identifier.uri https://hdl.handle.net/2292/57714
dc.description.abstract The commonly accepted kiwifruit harvest index based solely on soluble solids content (SSC) has lost its original significance owing to the increased sophistication of marketing coupled with the commercialisation of new cultivars, many with yellow flesh when ripe. The precision of novel harvest indices may be improved by a molecular understanding of fruit maturation changes in commonly monitored fruit attributes, including SSC, flesh colour and firmness. Transcriptional changes in the early-maturing Actinidia chinensis var. chinensis ‘Zesy002’ and later maturing A. chinensis var. deliciosa ‘Hayward’, grown at a single site, have been quantified weekly in the period leading up to and past the commercial harvest period. Transcriptomic data highlighted numerous maturation related changes in the fruit, and differences between the two cultivars. Examples of gene changes of relevance for non-structural carbohydrates included the large sub-unit 4 of ADP-glucose pyrophosphorylase (APL4) indicative of starch synthesis, BETA AMYLASE 3.2 (BAM3.2) for starch breakdown and the sugar transporter (SWEET9a). The association between STAYGREEN2 (SGR2) with flesh degreening was strong in ‘Zesy002’, although a later increase in ‘Hayward’ to levels similar to those in ‘Zesy002’ was not accompanied by an equivalent flesh colour change. In ‘Zesy002’ there were numerous individual cell wall gene changes associated with the change to rapid softening, including EXPANSIN7 (EXP7), POLYGALACTURONASE1 (PG1), PECTATE LYASE (PL), PECTIN METHYL ESTERASE (PME) and XYLOGLUCAN TRANSGLYCOSYLASE/ HYDROLASE (XTH). However, among these genes, it is possible to see similar changes in ‘Hayward’ which were not associated with a marked change in softening rate, including for EXP7 and PG1. The most obvious start points for changes in transcription of these genes were the seed coat colour change, the cessation of growth, and the change to ripening (rapid softening and starch breakdown). The findings are discussed with respect to fruit maturation and the possible use of gene markers as harvest indices.
dc.language en
dc.publisher Elsevier BV
dc.relation.ispartofseries Scientia Horticulturae
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm
dc.subject Science & Technology
dc.subject Life Sciences & Biomedicine
dc.subject Horticulture
dc.subject Agriculture
dc.subject Actinidia
dc.subject Kiwifruit
dc.subject Maturity
dc.subject Harvest index
dc.subject Markers
dc.subject XYLOGLUCAN ENDOTRANSGLYCOSYLASE ACTIVITY
dc.subject ACTINIDIA-CHINENSIS
dc.subject PECTIN METHYLESTERASE
dc.subject 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID
dc.subject HAYWARD KIWIFRUIT
dc.subject ETHYLENE RESPONSE
dc.subject SOLUBLE SOLIDS
dc.subject GREEN-RIPE
dc.subject CELL-WALLS
dc.subject TEMPERATURE
dc.subject 0601 Biochemistry and Cell Biology
dc.subject 0706 Horticultural Production
dc.title Transcriptomic analysis reveals differences in fruit maturation between two kiwifruit cultivars
dc.type Journal Article
dc.identifier.doi 10.1016/j.scienta.2021.110207
pubs.begin-page 110207
pubs.volume 286
dc.date.updated 2021-11-08T00:48:20Z
dc.rights.holder Copyright: The author en
pubs.author-url http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000656083600010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e41486220adb198d0efde5a3b153e7d
pubs.publication-status Published
dc.rights.accessrights http://purl.org/eprint/accessRights/RetrictedAccess en
pubs.subtype Article
pubs.subtype Journal
pubs.elements-id 853243
dc.identifier.eissn 1879-1018
pubs.number 110207


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